cDNA DNA 2b transgenic lines 2b transgenic line Jack infected with CMV-S 18-4 19-2 21-7 11-1 C C CP Actin 3 Supplementary Fig. 1 RT-PCR analysis for coat protein (CP) mRNA of CMV-S in the seed coat of 2b transgenic soybean. The seed coat samples were prepared from developing soybean seeds, and expression of the Actin 3 gene was examined as an invariant control. Open arrowheads indicate the positions of cDNA amplicons corresponding to each target sequence, whereas the filled arrowhead indicates that of genomic amplicons (DNA lanes). Lanes C correspond to a transgenic line harboring the control vector (pUHR).