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Protein purification robot centrifugation sonication aspirate/dispense 96x100ml fermentation tube PowerPoint Presentation
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Protein purification robot centrifugation sonication aspirate/dispense 96x100ml fermentation tube - PowerPoint PPT Presentation


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Crystallomics Core. Automated purification instrumentation. Protein purification robot centrifugation sonication aspirate/dispense 96x100ml fermentation tube soluble and insoluble fractions. Subsequent Ni-NTA purification. UEX3 A10-H10 Soulubles. Scott Lesley 3/12/01. HT crystallization.

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Protein purification robot centrifugation sonication aspirate/dispense 96x100ml fermentation tube


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    1. Crystallomics Core Automated purification instrumentation • Protein purification robot • centrifugation • sonication • aspirate/dispense • 96x100ml fermentation tube • soluble and insoluble fractions Subsequent Ni-NTA purification UEX3 A10-H10 Soulubles Scott Lesley 3/12/01

    2. HT crystallization Crystallomics Core 142,500 (target value) experiments/day (second generation robot) 50 to 100-fold reduction protein requirements Agincourt – second generation crystallization robot