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REGULACE GENOV É EXPRESE

REGULACE GENOV É EXPRESE. C E N T R A L D O G M A. I N M O L B I O L O G Y. Initiation is the rate-limiting step for translation  main target of the translational regulation. DNA transcription RNA translation - initiation - elongation - termination PROTEIN. P-site.

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REGULACE GENOV É EXPRESE

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  1. REGULACE GENOVÉ EXPRESE

  2. C E N T R A L D O G M A I N M O L B I O L O G Y

  3. Initiation is the rate-limiting step for translation  main target of the translational regulation DNAtranscriptionRNAtranslation - initiation- elongation- terminationPROTEIN

  4. P-site 5’ end determination / (f)Met-tRNAiMet recruitment / mRNA recruitment / the AUG start codon recognition • BACTERIA • Shine-Dalgarno seq. (AGGAGG motif) in every mRNA complementary to 3’ end of 16S rRNA (small subunit) • 3 so called initiation factors (IFs) => fMet-tRNAiMet recruitment • EUKARYOTS • 5’ 7Methyl-Guanosine Cap • 3’ Poly-tail • 12 eukaryotic initiation factors (eIFs) • Scanning for AUG

  5.  General Translation Initiation pathway and its regulation

  6. Main Object of our Studies => roles of eIFs 1, 2, 3 and 5 in TI • contacts of the eIFs with the 40S ribosome => structure & function • role of eIF3 and its individual subunits in Met-tRNAiMet binding as well as in scanning and AUG recognition • role of eIF3 in TI on the Hepatitis Virus C • model organism budding yeast S. cerevisiae • genetics • molecular biology • biochemistry How does the cell make sure that all proteins are translated in a timely and spatially coordinated fashion?

  7. Anna DelijannisováAnna HerrmannováMartina JanoškováOlga JanouškováEdit RutkaiBéla Szamecz Leoš Valášek(valasekl@biomed.cas.cz)Institute of MicrobiologyAS CR, Prague Alan G. HinnebuschNICHD, USAPeter Lukavsky,MRC Cambridge, UK Klaus H. NielsenUNI of Aarhus, DKMartin Pospisek,Charles UNI, CRJon LorschJohn Hopkins UNI, USA

  8. Laboratoř regulace genové exprese Akademie věd České republiky hledá šikovné a pracovité zájemce o bakalářské a magisterské studium!!! nabízíme: a) zajímavá témata zaobírající se studiem jednoho ze základních procesů genové exprese => regulací proteosyntézy za použití modelového organismu „budding yeast“ Saccharomyces cerevisiae b) špičkově vybavenou laboratoř podporovanou zahraničními grantovými agenturami jako jsou: The Wellcome Trust (UK) Howard Hughes Medical Institute (USA) Fogarty International Center NIH (USA) c) publikační aktivitu v prestižních zahraničních časopisech d) práci v mladém, nadšením sršícím mezinárodním kolektivu požadujeme: a) zvídavého, bystrého, pracovitého a férového ducha b) intermediate english communication skills Kontantní adresa je uvedena níže. Díky, L. Valášek Leoš Valášek, Ph.D. Laboratoř regulace genové expreseMikrobiologický Ústav, AV ČRVídeňská 1083Praha, 142 20Tel:   +420 241 062 288Cell:  +420 724 731 661Fax:   +420 241 062 665e-mail: valasekl@biomed.cas.czhttp://www.veravalaskova.net/LV.html

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