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Bacterial viruses. Very complex shape, requiring 20 gene products for assembly - Capsid (head), contains linear dsDNA genome - Tail , consists of sheath plus internal tube. Bacteriophage T4. Figure 11.2.

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Presentation Transcript
slide2
Very complex shape, requiring 20 gene products for assembly

- Capsid (head), contains linear dsDNA genome

- Tail, consists of sheath plus internal tube

Bacteriophage T4

Figure 11.2

slide5
Phage T4 binds to E. coli by contact between its tail fibers and the outer membrane.

- A conformational change causes sheath to contract and inject the genome into the cell.

Bacteriophage T4

Figure 11.4

fig 10 15

Fig. 10-15

Nucleases

DNA polymerase

New sigma factors

Mature phage particle

Tail, collar, base

plate, and tail

fiber proteins

Phage

head

proteins

Phage DNA

T4 lysozyme

production

Phage DNA replication

Infection

Early mRNA

Middle mRNA

Late mRNA

Self assembly

Middle proteins

Late proteins

Early proteins

Lysis

0

5

10

15

20

25

Minutes

slide8
Progeny genomes are linked in a concatemer.

- Cut with an offset, so that individual linear genomes have slight overlaps

Figure 11.7

slide15
Progeny genomes are linked in a concatemer.

- Cut with an offset, so that individual linear genomes have slight overlaps

Figure 11.7

slide16
Each phage particle is assembled by convergence of three pathways involving head, tail, and tail fibers.

Figure 11.8