Utilisation des systèmes photosynthétiques comme éléments
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Utilisation des systèmes photosynthétiques comme éléments. biologiques pour la détection de polluants. stroma. granum. thylacoïde. chloroplaste. lamelle stromatique. membrane thylacoïde. thylacoïde. espace thylacoïde ou lumen. HERBICIDES. stroma. CF. FNR. Fd. 1. cyt b 559. LHC II.

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Polluants

Utilisation des systèmes photosynthétiques comme éléments

biologiques pour la détection de polluants

stroma

granum

thylacoïde

chloroplaste

lamelle stromatique

membrane thylacoïde

thylacoïde

espace thylacoïde ou lumen

HERBICIDES

stroma

CF

FNR

Fd

1

cyt b 559

LHC II

LHC I

pool PQ

PS II

cyt b

PS I

CF

0

f

membrane thylacoïde

6

PCy

-

+

lumen

2 H

O

OEC

O

+ 4 e

+ 4 H

2

2

Polluants

:

herbicides

de la famille des urées et de la famille des triazines

inhibent la photosynthèse en agissant sur le transfert d'électrons au niveau

du photosystème II (PSII) et

métaux lourds

qui agissent à différents

endroits avec une sensibilité plus marquée du PSII


Polluants

4) Open and Close circuit éléments

We realised works to see the differences between the inhibition with an open circuit (big quantities of herbicides) and a close one (5 mL)

Peristaltic pump

Peristaltic pump

Herbicide Cuve

Herbicide Cuve

Hansatech + photosynthetic system

Hansatech + photosynthetic system

End of circuit

Computer

Computer

Close circuit

Open circuit


Polluants

This table represents the values obtains with the two kinds of circuit

Increase of inhibition: 25 %


Polluants

Inhibition curve of diuron with continuous flow of circuit

We wanted to determinate:

- I50 at 15 min :

-

- I10 at 15 min :

46 ppb

-I50 at 30 min :

8 ppm

-I10 at 30 min :

5 ppb

This method is not enough sensitive

Values higher than the European limits


Polluants

Inhibition curve of different herbicides (manual method) of circuit

You are going to see, the inhibition curves of:

- Diuron

- Atrazine

- Metribuzine