76 151 25 226 50 301 75 376 100 451 125 526 150 601 175 676 200 751 225 826 250 901 275 976 300

1. gttgaaagatcgaaagtttactatatttatacatacattattatttttcactcttttgtctaaaaacgtaatcatc ATGTTACTGGAAAAGATTGTGGACGTAATCTCTGGGAAAAATGACGATGGAAAAAAGATGAAAGGAACTGTTGTG M L L E K I V D V I S G K N D D G K K M K G T V V TTGATGAAGAAGAATGCATTGGACTTTAATGATGTCAATGCTTCTTTTCTTGATGGAGTTCTTGAGTTCCTTGGC L M K K N A L D F N D V N A S F L D G V L E F L G AAGAGAGTCTCTTTGCAGTTGATCAGCTCTGTTCATGGTGATCCTGCGAATGGTTTACAAGGGAAACGTAGCAAG K R V S L Q L I S S V H G D P A N G L Q G K R S K CCAGCTTACTTGGAGAACTGGCTCACTACGCGAACCCCATTAGTCGCAGGCGAATCAGCCTTTGATGTCACGTTT P A Y L E N W L T T R T P L V A G E S A F D V T F GATTGGGACGAGGATATTGGAGTTCCAGGGGCATTTATCATCAATAATTTGCACTTCAATGAGTTTTTCCTTAAA D W D E D I G V P G A F I I N N L H F N E F F L K TCACTCACTCTTGAAGACGTTCCCAATCATGGCAAGATTCATTTTGTCTGTAATTCTTGGGTTTATCCTGCTAAA S L T L E D V P N H G K I H F V C N S W V Y P A K AGATACAAATCAGAACGGATTTTCTTTGCTAATCAGGCATATCTTCCCCACGAAACTCCAGAACCATTGCGCGAA R Y K S E R I F F A N Q A Y L P H E T P E P L R E TACAGAGAAAAAGAATTAGTGACCTTAAGAGGAGATGGAAATGGAAAGCTTGAGGAATGGGACAGGGTTTATGAC Y R E K E L V T L R G D G N G K L E E W D R V Y D TATGCTTTCTACAACGACTTGGGTGATCCAGAAAGAGGCGAAGCGTATGCTAGGACTATCTTGGGAGGATCTGCT Y A F Y N D L G D P E R G E A Y A R T I L G G S A GAGTTCCCATACCCTCGGAGAGGAAGAACAGGCAGAAAGTCAACAAAAGCAGATCCTAAAAGTGAAAGTAGGATT E F P Y P R R G R T G R K S T K A D P K S E S R I CCATTGCTTATGAGTTTAGACATCTATGTACCAAGAGATGAGCGTTTTGGACACATTAAGTTGTCGGACTTCCCG P L L M S L D I Y V P R D E R F G H I K L S D F P ACATATGCTTTGAAATCCATTGTTCAGTTCCTTATCCCCGAGTTTCAGGCTCTCTTTGATAGCACTCCTGGTGAG T Y A L K S I V Q F L I P E F Q A L F D S T P G E TTTGACAGCTTTGAGGATGTACTGAGGCTTTATGAAGGAGGAATCAAATTGCCGCAAGGCCCTTTTCTCAAAGCC F D S F E D V L R L Y E G G I K L P Q G P F L K A CTCACTGACAGCATTCCTCTATCGATTCTAAAAGAAATCATCCGAACTGATGGTGAAGGGAAATTCAAATTCCCA L T D S I P L S I L K E I I R T D G E G K F K F P ACTCCTCAAGTCATTCAAGCGGATAAAAGTTCATGGAGGACTGATGAAGAATTTGCAAGAGAAATGCTTGCCGGA T P Q V I Q A D K S S W R T D E E F A R E M L A G GTAAATCCTGTCATAATCAGCAGACTCCAAGAGTTCCCTCCAAAAAGCAAGCTAGATACTGAAGTATATGGAAAC V N P V I I S R L Q E F P P K S K L D T E V Y G N CAAAACAGTACAATAACCAAAGAACATATAGAGAATGCACTGGATGGGCTAACTATCGATGATGCAATCAAGACA Q N S T I T K E H I E N A L D G L T I D D A I K T AACAGGCTTTACATATTAAACCATCATGACATGCTTATGCCGTATGTGAGGAGAATAAACACGACAAACACAAAA N R L Y I L N H H D M L M P Y V R R I N T T N T K CTCTACGCCTCAAGAACTCTGCTTTTCTTGCAAGACGATGGAACAATGAAGCCAATAGCAATTGAACTAAGCTTG L Y A S R T L L F L Q D D G T M K P I A I E L S L CCACATCCGGATGGAGATGAACTTGGGGCTGTTAGCAAAGTTTATACCCCAGCCGATCGAGATGTTGAGGGTACG P H P D G D E L G A V S K V Y T P A D R D V E G T ATCTGGCAATTGGCTAAAGCTTATGTTGCAGTGAATGACTCGGGTGTTCATCAGCTAATCAGTCACTGGTTGAAT I W Q L A K A Y V A V N D S G V H Q L I S H W L N ACACATGCAGCAATTGAGCCGTTTGTGATTGCAACAAACAGGCAACTAAGCGTGCTTCACCCGATTCATAAACTT T H A A I E P F V I A T N R Q L S V L H P I H K L TTACATCCTCATTTTCGGGACACGATGAACATAAACGCTTTGGCAAGACAGATCTTAATCAATGCTGGTGGAGTT L H P H F R D T M N I N A L A R Q I L I N A G G V CTTGAGCTGACAGTTTTTCCTTCCAAATATGCGATGGAAATGTCTGCTGTAGTTTACAGAAATTGGGTCTTCCCT L E L T V F P S K Y A M E M S A V V Y R N W V F P GAACAAGCACTTCCGGTTGATCTCGTTAAGAGAGGAGTTGCAGTAGAGGACTCGAGTTCCCCACATGGCGTTCGC E Q A L P V D L V K R G V A V E D S S S P H G V R TTACTAATTCAAGACTACCCATACGCTGTTGATGGTTTAGAAATATGGTCAGCAATCAAAATTTGGGTAACAGAA L L I Q D Y P Y A V D G L E I W S A I K I W V T E TATTGCAACTTCTATTATAAATCAGATGAATCAGTTCTGAAAGATGATGAACTCCAAGCCTGGTGGAAAGAAGTT Y C N F Y Y K S D E S V L K D D E L Q A W W K E V CGGGAAGAAGGGCATGGTGACAAGAAAGATGAACCCTGGTGGCCTAAAATGCAAACACGTCAAGAGCTAATAGAT R E E G H G D K K D E P W W P K M Q T R Q E L I D TCTTGCACCATTATTATTTGGATAGCATCAGCACTTCATGCAGCAGTCAATTTTGGGCAATACCCTTATGCAGGT S C T I I I W I A S A L H A A V N F G Q Y P Y A G TACCTCCCAAATCGCCCAACATTAAGTCGAAGATTCATGCCTGAGCCAGGAACTCCTGAGTATGAAGAACTTAAG Y L P N R P T L S R R F M P E P G T P E Y E E L K ACAAATCCTGATCTGGCATACTTGAAAACAATCACTCCTCAACTGCAGACATTACTAGGAATTTCTCTCATAGAG T N P D L A Y L K T I T P Q L Q T L L G I S L I E ATATTGTCAAGGCATACATCAGATGAGGTTTACCTTGGACAGAGAGACTCATCTGAATGGACAAAGGACCAAGAA I L S R H T S D E V Y L G Q R D S S E W T K D Q E CCTCTTGCTGCTTTTGAGAGGTTTGGGAAAAAGTTGAGTGAAATCGAGGATCAAATTGTACAGATGAATGGCGAT P L A A F E R F G K K L S E I E D Q I V Q M N G D GAGAATTGGAAAAATAGGTCGGGGCCTGTTAAGGTTCCATATACGTTGCTCTTTCCTACAAGTGAAGAAGGACTC E N W K N R S G P V K V P Y T L L F P T S E E G L ACAGGCAAAGGAATACCCAACAGTGTGTCGATATAGtactttcatttcaatcttcatggtcaagtttcattttta T G K G I P N S V S I * Ttctttcttttgttcaattacggtgtcaagtttcattttccttcttttgttcaattacagtgtcaagttagtgtg atgtaatttttgtttgataaactttgtaatatttctcttgcaagcaagttaaaaaaaaaaaaaaaaaaaa 76 151 25 226 50 301 75 376 100 451 125 526 150 601 175 676 200 751 225 826 250 901 275 976 300 1051 325 1126 350 1201 375 1276 400 1351 425 1426 450 1501 475 1576 500 1651 525 1726 550 1801 575 1876 600 1951 625 2026 650 2101 625 2176 650 2251 675 2326 700 2401 725 2476 750 2551 775 2626 800 2701 812 2776 2846

2. Supplemental Figure S1. Nucleotide and derived amino acid sequences of CaLOX1 cDNA encoding pepper LOX. The deduced amino acid sequences are given below the nucleotide sequences. The transcriptional start site is in bold and the translation termination codon is marked by an asterisk (*). The PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain, or LH2 (lipoxygenase homology) domain, is underlined and the LOX domain is shaded in gray.