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O que acontece com o artigo após a submissão Osvaldo N. Oliveira Jr. email@example.com Instituto de Física de São Carlos, USP Núcleo Interinstitucional de Lingüística Computacional http://nilc.icmc.usp.br. A primeira barreira Editor recebe artigo
Osvaldo N. OliveiraJr.
Instituto de Física de São Carlos, USP
Núcleo Interinstitucional de Lingüística Computacionalhttp://nilc.icmc.usp.br
Com recomendação de “major revision”, decisão cabe a editor
Revistas de maior impacto mais exigentes
Please find attached a revised version of the manuscript BIOS-D-09-00619, which we submitted to Biosensors & Bioelectronics. We have taken onboard all the reviewers’ comments and modified the manuscript accordingly. Furthermore, we reduced the number of figures to fit the manuscript to the format of a Short Communication. The changes made are highlighted in red in the text. A supplementary file containing three figures was created, according to the suggestions proposed by reviewer 1, and they are properly indicated in the text. Also attached is a response sheet to the Reviewers’ comments.
:In hindsight we noted that we were not entirely clear about the correlation of the results from the monolayer experiments and those from the experiments with liposomes. In the revised manuscript we have tried to clarify these points, by including the following statements: …..
c) Figure 2. How the segregation of the peptide immersed in the bilayer is explained?Apparently it is forced by the lateral pressure. Is this process reversible?
The reviewer is absolutely correct in the explanation for the segregation, which is due to the lateral pressure. Moreover, the process is reversible as subsequent surface pressure-area isotherms are identical to the first one. These pieces of information have been added to the revised version.
There is some incomplete analysis that should be offered before introducing the model. For instance, the disappearance of the plateaus cause by SIT 2 in DOG by the inclusion of OCoA is not explained. They offer a model which needs more experimental details such as surface pressure in terms of the molar fraction of OCoA and DOG at constant SIT 2.
Response: We have performed further experiments with mixed monolayers of OCoA and DOG, without SIT2 and with a fixed concentration of SIT2, as suggested by the reviewer. The new data are included as Figure 5, together with a discussion on the data and on the disappearance of the plateaus.
At this point we should thank the reviewer for the suggestion of making the experiments with distinct relative concentrations of OCoA and DOG. As the description of Figure 5 and 6 indicates, new features appeared for the synergistic interaction between SIT2 and the mixture of the lipids. In addition to enhancing the quality of the discussion in the present manuscript, as we believe they did, the new findings opened the way for further studies, which we shall try and do in the future.
Please find attached a revised version of the manuscript “Interaction of a C-terminal peptide of Bos taurus diacylglycerol acyltransferase 1 with model membranes”, which we submitted for publication in BBA: Biomembranes. We decided to resubmit because all the points raised by the reviewers could be considered and we believe be addressed satisfactorily. For addressing the reviewers’ concerns we have performed two new series of experiments, and the results are commented upon in the revised manuscript. All the changes made in the manuscript are marked in yellow.
Also attached is a response sheet to the reviewers’ comments. The reply was made point by point, in order to stress that every single concern was addressed.
2) What is the effect of CoA in liposomes? (structure, fluidity, average vesicle size...)
The question is an interesting one, though we did not address it as our main object was to verify effects from SIT2. Because a fully-fledged study may be necessary to assess possible effects from CoA, we believe that it is out of the scope of the present manuscript.
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