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Antimicrobial Susceptibility test. testing sensitivity of the pathogen to specific antibiotics in order to choose best antimicrobial therapy. judged by determination of MIC and MBC. Minimum Inhibitory Concentration (MIC):

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antimicrobial susceptibility test
Antimicrobial Susceptibility test
  • testing sensitivity of the pathogen to specific antibiotics in order to choose best antimicrobial therapy.
  • judged by determination of MIC and MBC.
  • Minimum Inhibitory Concentration (MIC):
      • laboratory term that defines the lowest concentration (μg/mL) of the antibiotic that is able to inhibit growth of the microorganism.
  • Minimum Bacteriosidal Concentration (MBC):
      • laboratory term that defines the lowest concentration (μg/mL) of the antibiotic that is able to kill 99% of the organism.
antimicrobial susceptibility test1
Antimicrobial Susceptibility test

Zone of growth inhibition

  • Common methods
  • Disk diffusion test:
    • The antibiotic diffuses from an impregnated paper disc into an agar medium on which the test organism is growing.

Disk diffusion test

Kirby – Bauer test

antimicrobial susceptibility test2
Antimicrobial susceptibility test
  • E-test
    • The antibiotic diffuses from an impregnated strip containing gradient concentration an agar medium on which the test organism is growing.
antimicrobial susceptibility test3
Antimicrobial susceptibility test
  • Agar incorporation assays
    • The antibiotic dilutions are incorporated in an agar medium and spot inoculated with a number of test organisms.

Incubation

antimicrobial susceptibility test4
Antimicrobial susceptibility test

16

8

4

0

64

32

Antibiotic concentration mg/ml

  • Broth dilution assays
    • Serial dilutions of the antibiotic in growth medium are inoculated with the test organism.
  • Automated Systems: VITEK.