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Sherrie Jean, DVM Vanessa Lee, DVM Emory University School of Medicine

Sherrie Jean, DVM Vanessa Lee, DVM Emory University School of Medicine Division of Animal Resources Prachi Sharma, DVM, PhD Yerkes National Primate Research Center. Case 45, Slide 2008-5. Signalment 10-11 week old male Sprague-Dawley rat ( Rattus norvegicus ) Clinical history:

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Sherrie Jean, DVM Vanessa Lee, DVM Emory University School of Medicine

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  1. Sherrie Jean, DVM Vanessa Lee, DVM Emory University School of Medicine Division of Animal Resources Prachi Sharma, DVM, PhD Yerkes National Primate Research Center Case 45, Slide 2008-5 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  2. Signalment • 10-11 week old male Sprague-Dawley rat (Rattus norvegicus) • Clinical history: • Surgery: left lung lobe transplant on day 0 • No prior experiments or treatments • Normal recovery and post-operative period Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  3. Day 1 post-op: eating and drinking normally • Day 3 post-op: slightly tachypneic, lethargic and thin, but normal feces were present in the cage • Day 5 post-op: severely dyspneic, thin and had a few dehydrated fecal pellets in the cage • Day 7 post-op: Euthanized Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  4. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  5. Gross Pathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  6. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  7. Gross Pathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  8. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  9. Histopathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  10. Histopathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  11. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  12. Lung cultures: • Heavy growth of Pseudomonas aeruginosa and Enterococcus spp. • Liver cultures: • Scant growth of alpha hemolytic Streptococci Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  13. Left lung, severe diffuse subacute suppurative pulmonary necrosis and fibrosis with intralesional bacteria • Right thoracic and abdominal wall, focal hematoma with intramuscular hemorrhage • Left thoracic wall, focal extensive subacute necrosuppurative myositis and cellulitis Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  14. Intra- or post-operative contamination • Strict aseptic technique • Highest risk of transplant rejection and/or infection • Bronchiolitis obliterans • Bruising and excessive hemorrhage. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  15. Deuse T, Schrepfer S, Reichenspurner H, Hoyt G, Fischbein MP, Robbins RC, Pelletier MP. 2007. Techniques for experimental heterotopic and orthotopic tracheal transplantations - When to use which model? Transplant Immunology. 17: 255-261. • Dishop MK, Mallory GB, White FV. 2008. Pediatric Lung Transplantation: Perspectives for the Pathologist. Pediatr Dev Pathol. Jan 29:1. • Hirschburger M, Greschus S, Kuchenbuch T, Plötz C, Obert M, Traupe H, Padberg W, Grau V. Lung transplantation in the Fischer 344-->Wistar Kyoto rat strain combination is not suitable to study bronchiolitis obliterans. 2007. J Heart Lung Transplant. Apr 26(4):390-8. • Mattner F, Fischer S, Weissbrodt H, Chaberny IF, Sohr D, Gottlieb J, Welte T, Henke-Gendo C, Gastmeier P, Strueber M. 2007. Post-operative nosocomial infections after lung and heart transplantation. J Heart Lung Transplant. 26(3):241-9. • Yamane M, Sano Y, Nagahiro I, Aoe M, Date H, Ando A, Shimizu N. 2002. Humoral immune responses during acute rejection in rat lung transplantation. Transplant Immunology. 11: 31-37. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  16. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

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