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Advanced AFM Techniques for Analyzing CD Surfaces and Calibration Gratings

This protocol outlines the advanced techniques used in atomic force microscopy (AFM) for analyzing CD surfaces and calibration gratings. It includes steps for lowering cantilevers, positioning lasers, and selecting cantilevers with the appropriate stiffness. The procedure emphasizes the importance of fine-tuning laser locations and monitoring photodetector voltage for accurate data collection. With a focus on practical tips and troubleshooting (including manual reference), this guide showcases the meticulous process required to observe and analyze the pits and lands of a CD.

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Advanced AFM Techniques for Analyzing CD Surfaces and Calibration Gratings

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Presentation Transcript

  1. Protocol review

  2. Lowering the cantilever onto the sample surface

  3. Positioning the laser on the reflective surface of the cantilever

  4. Discussing how the cantilever interacts with the sample surface

  5. Selecting the cantilever with the appropriate stiffness

  6. When all else fails, read the manual.

  7. Looking up the specifications of our cantilever

  8. Our cantilever magnified ~1,000 times

  9. Tyree replaces the AFM head.

  10. Making predictions about the grating

  11. Learning how to fine-tune the location of the laser beam

  12. A close-up of laser adjustment

  13. Tramel keeps an eye on the photodetector voltage.

  14. Tyree finally gets the laser aimed at the right spot on the cantilever.

  15. Tramel finds out that it’s not as easy as it looks.

  16. Watching the AFM scan the surface of the calibration grating

  17. The grating looks good; the AFM is working properly.

  18. Tramel poses for GQ

  19. Analyzing the data from the calibration grating

  20. Pipetting sodium hydroxide to dissolve the label and acrylic layers

  21. Submerging the CD in sodium hydroxide

  22. Using duct tape to remove the label, acrylic and reflective layers

  23. Cleaning the surface

  24. Tyree has sacrificed part of his Celine Dion collection to science.

  25. Tyree positions the CD under the AFM.

  26. The tip approaches the CD surface.

  27. Blank tracks on a CD-R

  28. Finally, we see some information on a CD!

  29. Pits and lands on an audio CD

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