Introduction. Tissue culture is method of isolation and inoculation of tissues in artificial medium under in vitro condition. Banana is major fruit crop in India and Maharashtra stands first in banana production and productivity in India.
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Steps in Banana shoot tip culture
Young sucker for micro-propagation
Sword (left) and water sucker (right)
View of mother plant and sucker
Suckers separated from mother plant
Sterilization of explants in chamber
Removal of subsequent layers from the suckers and sterilization for banana shoot tip culture
Initiation of banana shoot after one week of inoculation
B. Sub cultured explants in growth room
Well grown banana shoots after two weeks of sub culturing on MS medium
Root development in banana shoots after transfer to rooting medium