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Genetic and Cellular Mechanisms of Pattern Formation

Genetic and Cellular Mechanisms of Pattern Formation. Neighboring cells instruct other cells to form particular structures: cell signaling and induction in the nematode Introduction

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Genetic and Cellular Mechanisms of Pattern Formation

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  1. Genetic and Cellular Mechanisms of Pattern Formation • Neighboring cells instruct other cells to form particular structures: cell signaling and induction in the nematode • Introduction • Induction: recall that this is involves some sort of communication between cells, either molecularly or by contact, to cause cellular differentiation by activating genes. • Induction in Vulval Development and C. elegans • C. elegans is a kind of “worm”, a nematode. • The vulva, the structure through which eggs are laid, has been studied. • In its larval stage, C. elegans has 6 cells in the area that will develop into the vulva. • What is called “the anchor cell” will release inducer molecules that will cause changes in the other vulval precursor cells.

  2. Genetic and Cellular Mechanisms of Pattern Formation • Induction in Vulval Development and C. elegans (cont’d) • Receptors in the vulva precursor cells bind to the inducer. • The cell closest to the anchor cell, receiving the highest amount of inducer, divides and differentiates to form the inner part of the vulva and also produces a second inducer. • Other vulval precursor cells have receptors for this second inducer, bind to the inducer, divide and become outer vulva cells. • This cascade of induction can be found in the formation of many other organs, in many other animals, all by signal transduction pathways.

  3. Figure 21.17 Cell signaling and induction in the development of the nematode vulva

  4. Genetic and Cellular Mechanisms of Pattern Formation • Programmed Cell Death (Apoptosis) • Definition: timely cell suicide during organismal development • Suicide proteins are produced that cause the cells to breakdown, die and then are engulfed by neighboring cells. • Genes and Proteins Involved in C. elegans • In C. elegans there are two apoptosis genes, ced-3 and ced-4, coding for proteins Ced-3 and Ced-4. These proteins are kept in the inactivated form in the cell (so they are stored) • Ced-9 regulates Ced-3 and Ced-4. • These proteins all activate nucleases and proteases

  5. Genetic and Cellular Mechanisms of Pattern Formation • Programmed Cell Death (cont’d) • Apoptosis in Humans • It is thought that apoptosis proteins cause the mitochondrial membrane to leak which releases other self-destructive proteins. • One such molecule is cytochrome c which is part of the electron transport chain. • Important in: • Formation of fingers and toes • Normal operation of the immune system when cells kill viral infected cells.

  6. Figure 21.18 Apoptosis (programmed cell death)

  7. Genetic and Cellular Mechanisms of Pattern Formation • Plant development depends on cell signaling and transcriptional regulation • Introduction • Cell signaling could not rely on cellular movement because of the rigid cell walls. • Plant morphogenesis or the taking of shape relies on the plane in which a cell divides. • Plants are similar to animals in relying on induction and regulating transcription. • Arabidopsis has been the main plant used for these studies.

  8. Genetic and Cellular Mechanisms of Pattern Formation • Cell Signaling in Flower Development • Plants will develop flowers at certain times based on temperature, length of night and so these environmental cues affect cell signaling. • A floral meristem: this is a growing region of a plant that will develop into a flower. • The floral meristem has 3 layers to it. • The flower that will be produced has very different parts: petals, egg-containing carpels, stamens which bear the anthers with pollen and usually green sepals which cover the petals when the flower is closed. • Researchers found that chimeras, organisms that were made of genetically different cells, like one of the cell layers was from one plant and another cell layer from another, had floral development controlled by one of the cell layers (layer 3 or the innermost)

  9. Figure 21.19 Induction in flower development

  10. Genetic and Cellular Mechanisms of Pattern Formation • Organ Identity Genes in Plants (Arabidopsis) • Organ Identity Genes: these genes determine whether a petal or a stamen or some other organ will grow. • Mutations in an OIG can produce a petal where some other structure should be located. These OIG therefore are analogous to the homeotic genes in the fruit fly. • These OIG could be controlling a whole series of other genes to bring about the flower’s proper structure. • OIGs encode for transcription factors that bind to DNA

  11. Figure 21.20a Organ identity genes and pattern formation in flower development: Normal flower development

  12. Figure 21.20b Organ identity genes and pattern formation in flower development: In situ hybridization

  13. Figure 21.20c Organ identity genes and pattern formation in flower development: Organ identity mutants Last Lecture Slide

  14. Figure 21.x2a Laboratory mice: brachyury mutant

  15. Figure 21.x2b Laboratory mice: eye-bleb mutant

  16. Figure 21.x2c Laboratory mice: Hfh11 mutant

  17. Figure 21.x2d Laboratory mice: Lama2 mutant

  18. Figure 21.x2e Laboratory mice: Lepr mutant

  19. Figure 21.x2f1 Laboratory mice: Mgf mutant

  20. Figure 21.x2f2 Laboratory mice: Pax3 mutant

  21. Figure 21.x2g Laboratory mice: Otc mutant

  22. Figure 21.x2h Laboratory mice: Pax6 mutant

  23. Figure 21.x2i Laboratory mice: Pit1 mutant

  24. Figure 21.x2j Laboratory mice: pudgy mutant

  25. Figure 21.x2k Laboratory mice: ruby-eye mutant

  26. Figure 21.x2l Laboratory mice: stargazer mutant

  27. Figure 21.x2m1 Laboratory mice: ulnaless mutant

  28. Figure 21.x3 Nude mouse

  29. Figure 21.x4 Normal and double winged Drosophila

  30. Figure 21.x1 Drosophila eyes

  31. Figure 21.4 Cell lineage in C. elegans

  32. Figure 21.9 Determination and differentiation of muscle cells (Layer 1)

  33. Figure 21.9 Determination and differentiation of muscle cells (Layer 2)

  34. Figure 21.9 Determination and differentiation of muscle cells (Layer 3)

  35. Figure 21.x5 Mutant Drosophila eyes

  36. Figure 21.15 Homologous genes that affect pattern formation in a fruit fly and a mouse

  37. Figure 21.16 Homeobox-containing genes as switches

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