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GC. Schematic of a GC. Typical GC. Computer Controls for Method and Output . Carrier gas/ Regulator. Varian 3350 Gas Chromatograph. Separation. Flow of Mobile Phase. Injector. Detector. T=0. T=10’. T=20’. Most Interaction with Stationary Phase Least. GC – Peak Broadening.

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typical gc
Typical GC

Computer Controls for

Method and Output

Carrier gas/

Regulator

Varian 3350 Gas

Chromatograph

separation
Separation

Flow of Mobile Phase

Injector

Detector

T=0

T=10’

T=20’

MostInteraction with Stationary PhaseLeast

gc column efficiency
GC – Column Efficiency

Capillary

Packed

gc film thickness effect
GC – Film Thickness Effect

0.25mm

1.0mm

3.0mm

5.0mm

gc isothermal and programming
GC – Isothermal and Programming

500C 3min

To 1400C at

100C/min

1050C

500C

gc injectors28
GC - Injectors
  • SPLIT INJECTOR
  • Advantage - Limitations
  • Injected zone narrow
  • Small sample aliquot avoids overloading
  • Mass discrimination of sample components (different range of volatility)
  • Systematic errors for quantitative analysis
  • In trace analysis: only part of analytes to detector
gc injectors29
GC- Injectors
  • SPLITLESS INJECTOR
  • Advantage - Limitations
  • Avoids large tailing of solvent peak
  • Allows transfer of main part of sample components into detector
  • Trace analysis: favourable technique for insertion of diluted samples
  • Recondensation of solvent at top of capillary: possible damaging stationary phase
  • Only columns with chemically bonded phases
  • Necessary wettability of stationary phase for condensed solvent (droplets)
injection technique and peak discrimination
Injection Technique and Peak Discrimination

Filled Needle

Hot Needle

Cold On-Column

gc injectors33
GC - Injectors
  • Advantage of on-column injector:
  • avoids mass discrimination effects
  • trace analysis: enables quantitative insertion of sample into column (and detector)
  • labile components not stressed thermally
slide55

Fatty Acids

Marine Source

Animal Source

slide56

Conditions Column: SP-2560, 100m x 0.25mm ID, 0.20µm filmCat. No.: 24056Oven: 140°C (5 min) to 240°C at 4°C/min, hold 15 minCarrier: helium, 20cm/sec, 175°CDet.: FID, 260°CInj.: 1µL Supelco 37 Component FAME Mix (10mg/mL total), split (100:1), 250°C

List of components on the next slide

components for sp 2560 chromatogram
Analyte Data Component (acid methyl ester) Weight (%)1. C4:0 (Butryic) 42. C6:0 (Caproic) 43. C8:0 (Caprylic) 44. C10:0 (Capric) 45. C11:0 (Undecanoic) 26. C12:0 (Lauric) 47. C13:0 (Tridecanoic) 28. C14:0 (Myristic) 49. C14:1 (Myristoleic) 210. C15:0 (Pentadecanoic) 211. C15:1 (cis-10-Pentadecenoic) 212. C16:0 (Palmitic) 613. C16:1 (Palmitoleic) 214. C17:0 (Heptadecanoic) 215. C17:1 (cis-10-Heptadecenoic) 216. C18:0 (Stearic) 417. C18:1n9c (Oleic) 418. C18:1n9t (Elaidic) 219. C18:2n6c (Linoleic) 2

20. C18:2n6t (Linolelaidic) 221. C18:3n6 (g-Linolenic) 222. C18:3n3 (a-Linolenic) 223. C20:0 (Arachidic) 424. C20:1n9 (cis-11-Eicosenoic) 225. C20:2 (cis-11,14-Eicosadienoic) 226. C20:3n6 (cis-8,11,14-Eicosatrienoic) 227. C20:3n3 (cis-11,14,17-Eicosatrienoic) 228. C20:4n6 (Arachidonic) 229. C20:5n3

(cis-5,8,11,14,17-Eicosapentaenoic) 230. C21:0 (Henicosanoic) 231. C22:0 (Behenic) 432. C22:1n9 (Erucic) 233. C22:2 (cis-13,16-Docosadienoic) 234. C22:6n3

(cis-4,7,10,13,16,19-Docosahexaenoic) 235. C23:0 (Tricosanoic) 236. C24:0 (Lignoceric) 437. C24:1n9 (Nervonic) 2

Components for SP 2560 Chromatogram