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Exploring GO Annotations from Various Secondary Sources for Chick GAPDH Analysis

This resource delves into the acquisition of Gene Ontology (GO) annotations for the chicken GAPDH gene (P00356) from multiple secondary sources, including public databases like NCBI and UniProtKB, genome browsers like Ensembl, and array vendors such as Affymetrix. We highlight important considerations when accessing these resources, such as the authenticity of the original source, update frequency, and the presence of evidence codes. A comparison of GO annotations from QuickGO, the UniProtKB database, NCBI Entrez Gene, Ensembl, and others is also included, emphasizing the variability in evidence reporting.

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Exploring GO Annotations from Various Secondary Sources for Chick GAPDH Analysis

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  1. GO Annotation from different sources

  2. Secondary Sources of GO annotation • EXAMPLES: • public databases (eg. NCBI, UniProtKB) • genome browsers (eg. Ensembl) • array vendors (eg. Affymetrix) • CONSIDERATIONS: • What is the original source? • When was it last updated? • Are evidence codes displayed?

  3. C. Obtaining GO annotations from Secondary Sources AIM: Look at how different public resources and tools display GO.

  4. GO annotation obtained for chick GAPDH (P00356) from different sources: • QuickGO (primary source) • UniProtKB database • NCBI Entrez Gene page • Ensembl browser • Affymetrix chick array • g:profiler gene expression analysis tool

  5. QuickGO

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  8. Comparison of Chicken GAPDH GO annotation obtained from different sources. GO evidence codes not reported.

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