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Quorum Sensing System

Quorum Sensing System

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Quorum Sensing System

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  1. Quorum Sensing System LuxI and LuxR aiiA SenderとReceiverの選択ができるようにする Sender Receiver Sensitive Normal aiiA Receiver Strong(Super Sender) Normal

  2. Quorum Sensing System LuxI and LuxR aiiA SenderとReceiverの選択ができるようにする Sender Receiver Sensitive Normal aiiA Receiver Strong(Super Sender) Normal

  3. aiiAinverter Receiver LuxR GFP aiiA pTet pLux cI inverter Low AHL Low AHL aiiA GFP High AHL High AHL

  4. Senderの数とGFP発現 methionine metK S-adenosyl methionine LuxI Sender(BW25113 or XL10G), Methionine (10mM or 0mM) 3OC6HSL(AHL) Sender:5×10^8~5×10 Receiver

  5. AHL synthesis AHL synthesis methionine S-adenosyl methionine 3OC6HSL metK LuxI, acyl-ACP Sender(BW25113 or XL10G), Methionine (10mM or 0mM) Sender:5×10^8~5×10 Receiver