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Sterilization Methods. Microbiology Biology II Mrs. Hieneman. Terminology. Sterilization – complete elimination of microbial viability Sterilant – chemical agent that sterilizes Disinfection – killing, inhibition or removal of disease-causing microbes.

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sterilization methods

Sterilization Methods

Microbiology

Biology II

Mrs. Hieneman

terminology
Terminology
  • Sterilization – complete elimination of microbial viability
  • Sterilant – chemical agent that sterilizes
  • Disinfection – killing, inhibition or removal of disease-causing microbes.
  • Disinfectant – chemical agent that disinfects
  • Antiseptic – chemical agents applied to tissues which prevent sepsis
  • Sanitation – establishment of environmental conditions favorable to health.
terminology1
Terminology
  • -cide: to kill
    • Germacide
    • Bactericide
    • Fungicide
  • -static: to inhibit growth
    • Bacteriostatic
    • Fungistatic
oxygen requirements for microbes
Oxygen Requirements for Microbes
  • Obligate aerobes – grow only in the presence of oxygen.
  • Strict anaerobes – grow only in the absence of oxygen.
  • Facultative anaerobe – can grow in presence (best) or absence of oxygen.
  • Microaerophilic – require small amount of oxygen (about 5%), inhibited by normal (oxygen) of atmosphere.
  • Aerotolerant – oxygen?? Who cares!!!!
chemical agents
Chemical Agents
  • Work by disrupting cellular membranes, oxidizing macromolecules, denaturing proteins.
  • Include disinfectants and antiseptics
  • Classified base upon effectiveness:
    • High Level – effective against all life
    • Intermediate Level – defined as tuberculocidal, as well as more resistant viruses (hepatitis C virus)
    • Low Level – kill vegetative cells of bacteria and fungi and enveloped viruses.
chemical agents1
Chemical Agents
  • Phenolics – contain phenol or its derivatives
    • Disinfectant; intermediate to low level
  • Aldehydes
    • Formaldehyde
    • Disinfectant: high to intermediate level
  • Alcohols
    • Antiseptics and disinfectants; intermediate level
chemical agents2
Chemical Agents
  • Halogens
    • Disinfectant and antiseptic; intermediate level
  • Heavy Metals
    • Disinfectant and antiseptic; low level
  • Soaps
    • Detergents derived from lipids
    • Antiseptic and disinfectants; low level
  • Gases
    • Disinfectant; high level
what can be used in our lab
What Can Be Used In Our Lab????
  • Soap
  • Disinfectant
  • Antiseptic
  • Sterile filtration
  • Bunsen Burner
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