Comprehensive Analysis of Brine Microbial Communities at 1500m and 50m Depths
This study investigates the microbial communities in brine environments at depths of 1500m and 50m using advanced genomic approaches. We conducted multiple analyses including BLASTN and BLASTX for nucleotide and protein functions, identifying matched and unmatched sequences against KEGG pathways. Our methodology includes domain analysis using Pfam, as well as gene ontology comparisons with known brine pools. The assessment is carried out on robust workstations, aiding in a detailed understanding of microbial diversity and potential functionalities in extreme salinity conditions.
Comprehensive Analysis of Brine Microbial Communities at 1500m and 50m Depths
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Presentation Transcript
Accession Number (taxonomy) KEGG (pathway) Raw reads BLASTN (nt / env_nt) Done for brine, 1500m and 50m. Done for brine. BLASTX (nr / env_nr) Protein (function) Matched Unmatched KEGG Accession Number (taxonomy) Subtractions (BLASTN + BLASTP/X or TBLASTX) Done for brine, 1500m and 50m. Pfam PhyMM BLASTN done for brine, 1500m and 50m. Domains (prot. families) Pfam GO Comparisons to HOT/ALOHA and Mediterranean Brine Pools (accession subtraction or sequence subtraction ??) GO (onthology) X Shaheen / CBRC Analysis will be performed using Workstations / CBRC SGI / AUC Time-logic / VBI
KEGG (pathway) Assembly ORFs ORFfinder Running for brine. BLASTX (nr / env_nr) Protein (function) Matched Unmatched Accession Number (taxonomy) Pfam PhyMM Running for brine. Domains Pfam Comparisons to HOT/ALOHA and Mediterranean Brine Pools (accession subtraction or sequence subtraction ??) GO Running for brine. GO (onthology) Shaheen / CBRC Analysis will be performed using Workstations / CBRC SGI / AUC Time-logic / VBI
