Student Designed DNA Extraction Question: Can we extract DNA from _____________________________________? Hypothesis: If we try to extract DNA from _____________________________________ then we will be able to get DNA out of ______________________________________ as measured by whether or not we see the white DNA in the test tube Introduction: ____________________________ _________________________________________ _________________________________________
Mechanical Break DowN of cell Smashing fruit in the bag = mechanical break down of cell wall
Chemical Break Down of cell 10mL Extraction buffer contains: 1 mLSoap & 1/8 tsp Salt- breaks down lipids (fats) in cell membrane & nuclear membrane A dash of Meat Tenderizer- breaks down proteins
Chemical Separation Cold Alcohol- DNA does not like to be in alcohol, so it bunches up and precipitates out of solution
DNA Extraction Experiment • This is a qualitative experiment (no graph) you will be measuring whether or not DNA was successfully extracted from each material during multiple trials (may need to modify each trial to see what works best) • Think about procedures that will be different based on your materials (plant or animal cell? So what about mechanical break down?)
Must be approved today • Group must be stamped for question & hypothesis TODAY (Pre-AP: must have procedures stamped today, too) • You will make your own extraction buffer, must calculate total amount required. Recipe below: Recipe for 10mL solution: 10mL H2O 1mL dish soap 1/8 teaspoon salt 1 pinch of meat tenderizer Work on DNA crossword puzzle once approved
Closure • Draw a picture of the lab set up for one of your trials in the DNA lab. Label all materials and amounts.
Procedures & Data Table • Fix procedures • Copy onto template • Set up data table • Come get approval stamp for procedures/data table • Work on crossword puzzle to review for quiz
Closure • Draw a picture that visually represents your hypothesis for your DNA extraction experiment
If your group is approved through data table (3 stamps total)… • Come show Ms. D to head into lab! Do NOT pour anything back into front stock bottles (extraction buffer, alcohol). Take only what you need.
Once you are finished • Complete a 2-3 paragraph conclusion for your experiment. • Staple together & turn in today (order= lab template, conclusion) • Complete the DNA worksheet • If you finish everything early…play DNA review games with partner!