Arabidopsis ecotypes infected with fusarium oxysporum wt and a fosnf1 mutant
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Arabidopsis ecotypes infected with Fusarium oxysporum (WT) and a fosnf1 mutant. Cape Verde Col-0 EST. H 2 O. WT. fosnf1. GFP-labeled F. oxysporum colonizing the root xylem of Arabidopsis. II Hyphal transfer. I

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Arabidopsis ecotypes infected with fusarium oxysporum wt and a fosnf1 mutant l.jpg
Arabidopsis ecotypes infected with Fusarium oxysporum (WT) and a fosnf1 mutant

Cape Verde Col-0 EST

H2O

WT

fosnf1


Slide2 l.jpg

GFP-labeled F. oxysporum colonizing the root xylem of Arabidopsis


Slide3 l.jpg

II

Hyphal transfer

I

Cocultivation +

selection

+

Mono-conidial

isolation

III

Agrobacterium cells

+ Fungal spores (1x105)

IV

Single spore

culture

Transformants

(10-100 / plate)

Phenotype

characterization

Agrobacterium tumefaciens-Mediated Transformation of Fungi


Slide4 l.jpg

OCCURRENCE OF AVR-Pita IN THE USA FIELD ISOLATES OF M. grisea

B

C

F

G

H

A

D

E

BM5-11

BM1-24

75A11

CA205

CA305

75A49

75A10

LO1-4

LO3-8

82A1

85M2

A598

A264

A119

75A1

A347

85L2

85A7

74T3

49D

793

D1

S1

Distribution pattern of the AVR-Pita gene family among rice isolates of Magnaporthe grisea

Probe Fs


Slide5 l.jpg

PCR of marker

genes

PCR of marker

genes

DNA extraction from

pure cultures

of plant pathogens

DNA extraction from

diseased plant or

cultured isolate

Sequencing/Genotyping of marker genes

Pathogen Database

Database

searching

Database

building

Known phenotypes of pathogen isolates

Risk assessment & implementation of

control/eradication measures

Schematic diagram of the plant pathogen database