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Gene Expression Analysis Vector Design

This report presents a detailed description of a genetic expression vector design, including promoters, reporters, and terminators, for functional analysis studies. The vector contains various genetic elements for efficient gene expression in research applications.

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Gene Expression Analysis Vector Design

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  1. attL1 Reporter attL2 attL4 Promoter attR1 attR2 Terminator attL3 Promoter Entry Clone Reporter Entry Clone Terminator Entry Clone + + KanR KanR KanR + attR4 CmR-ccdB attR3 Destination Vector (AmpR CmR ccdB+) AmpR LR clonase attB4 Promoter attB1 Reporter attB2 Terminator attB3 Expression Clone (AmpR) AmpR + attP4 CmR-ccdB attP3 Plasmid By-product kanR attP1 kanR attP2 kanR

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