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Pyrosequencing reveals the diversity and variability of bacterial communities on speleothem surfaces in Kartchner C PowerPoint Presentation
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Pyrosequencing reveals the diversity and variability of bacterial communities on speleothem surfaces in Kartchner C - PowerPoint PPT Presentation


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Pyrosequencing reveals the diversity and variability of bacterial communities on speleothem surfaces in Kartchner Caverns. Marian Ortiz - PhD Student Soil, Water and Environmental Science Dept Dr. Raina Maier’s Laboratory. Introduction. Background

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Pyrosequencing reveals the diversity and variability of bacterial communities on speleothem surfaces in Kartchner Caverns

Marian Ortiz - PhD Student

Soil, Water and Environmental Science Dept

Dr. Raina Maier’s Laboratory

introduction
Introduction
  • Background
    • Kartchner Caverns is near Benson, Arizona
    • Discovered in 1974 and kept secret for 14 years
    • Cave is oligotrophic with elevated CO2 and high humidity
    • NSF Microbial Observatory
  • Objective
    • Determine the structure and diversity of cave microbial communities
  • Hypothesis
    • Kartchner Caverns provides a habitat for unique microbial communities
  • Relevance
    • Discovery of new bacteria
    • Potential for new antibiotics, biosurfactants and other important secondary metabolites
slide3

Kartchner Caverns

Swab Samples

DNA Extraction

  • Methodology
  • Results

PCR of V6 region of the

Bacterial 16S rRNA gene

454 Pyrosequencing

BW

SA

Analysis of Sequence Data

* Operational taxonomic units

bacterial phylogenetic distribution of two rooms in kartchner caverns
Bacterial phylogenetic distribution of two rooms in Kartchner Caverns

Big Wall room

Strawberry Alcove room

  • Conclusions
    • There is broad bacterial diversity in caves.
    • Different distribution of bacterial groups in different rooms of the cave, BUT…...