The effects of ampicillin on the growth of e coli
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The Effects of Ampicillin on the Growth of E. Coli. Michelle Anthony Khadija Lawrence . Escherichia coli . Gram-negative Rod shaped Pathogenic strains . http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg. Ampicillin .

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The effects of ampicillin on the growth of e coli

The Effects of Ampicillin on the Growth of E. Coli

Michelle Anthony

Khadija Lawrence


Escherichia coli
Escherichiacoli

  • Gram-negative

  • Rod shaped

  • Pathogenic strains

http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg


Ampicillin
Ampicillin

http://upload.wikimedia.org/wikipedia/commons/4/48/Ampicillin-2D-skeletal.png


Hypothesis
Hypothesis

  • The maximum growth of E. coli will be inhibited as the concentration of ampicillin is increased.


Components
Components

  • Culture (E. coli)

  • Ampicillin

  • 10x lower LB

  • H2O


Experiments 1 4
Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 1 41
Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 1 42
Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 5 10
Experiments 5-10

  • No titrations

    • Altered amounts of ampicillin

  • Varied the ranges each week


Experiments 5 101
Experiments 5-10

  • No titrations

    • Altered amounts of ampicillin

  • Varied the ranges each week

  • Results from each week lead to

    final experiment


Experimental design
Experimental Design

* All values in table are in microL


3

0

5

9

12

16


Conclusion derived from results
Conclusion derived from Results

  • Maximum growth was not inhibited

    • Normal curve max: 1.088

    • Highest amp max: 1.044

  • Lag phase dramatically affected after 16microL is exceeded

  • IC50 not determined

  • Ampicillin affects length of lag phase

    • Graph consistent with scale


References
References

  • http://www.cdc.gov/ecoli/general/

  • http://www.foodsafety.gov/poisoning/causes/bacteriaviruses/ecoli/

  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1583696/?page=4


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