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Ecotossicologia e sicurezza ambientale dei sedimenti fitorimediati

Analysis of EU, Italian, and Spanish regulations on the use of dredged sediment-based substrates in agriculture. Assessment of sediment eco-toxicology and risk analysis based on recent regulatory references.

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Ecotossicologia e sicurezza ambientale dei sedimenti fitorimediati

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  1. Ecotossicologia e sicurezza ambientale dei sedimenti fitorimediati Giancarlo Renella dispaa, universita’ degli studi di firenze

  2. - The end - A1.1 - A review of the EU and Italian and Spanish regulations/laws regarding the use of dredged sediment based substrates in agriculture being in force at the date of the project beginning; Action B.1: Analysis of sediment eco-toxicology

  3. Bonifica e analisi di rischio: riferimentinormativirecenti

  4. Italian regulation on dredged sediments Italianwastelegislation LG.D. 152/2006 - part IV, and amendaments. Dredged sediments: • Exclusion from the scope of waste legislation (Art. 185) • Waste • Ending to be waste (Art. 184-ter, quarter) • By-product (Art. 184-bis) Italian water legislation LG.D. 152/2006 - part III, as amended by M.D. 260/2010 • Goodwater status • Water Protection Plan of the river basin district • Annulsedimentsmonitoring for some substances Legislation on SIN L. 84/1994, Art, 5-bis, L. 27/2012, L. 221/2015, M.D. 7 Nov. 2008, M.D. 4 Aug. 2010. Dredged sediments: • Immersion atsea, reuse in water • Use on land • Storage in CDF • Outside SIN: Manual for marine sediments management’ (APAT, ICRAM, 2007) Legislation on excavated soil and rocks - M.D. 161/2012. • DS eligible to be used directly ; pollutants below C.S.C. • Re-use:nourishments, land improvements, restoration, road embankments, industrial production processes as quarry materials.

  5. Bonifica e analisi di rischio: riferimentinormativirecenti

  6. Bonifica e analisi di rischio: riferimentinormativirecenti

  7. Bonifica e analisi di rischio: riferimentinormativirecenti

  8. Bonifica e analisi di rischio: riferimentinormativirecenti

  9. Bonifica e analisi di rischio: riferimentinormativirecenti

  10. Bonifica e analisi di rischio: riferimentinormativirecenti

  11. Bonifica e analisi di rischio: riferimentinormativirecenti

  12. Bonifica e analisi di rischio: riferimentinormativirecenti

  13. Bonifica e analisi di rischio: riferimentinormativirecenti

  14. Bonifica e analisi di rischio: riferimentinormativirecenti

  15. Ecotossicologia: fattori chiave dell’approccio TRIAD Concentrazione totale Biomarker Saggi biologici Biodisponibilità Parametri ecologici

  16. Ecotossicologia: test quantitativi La tossicità dei sedimenti fitorimediati è stata valutata prima e dopo la crescita delle piante mediante una batteria di bioindicatori, al fine di analizzare il più ampio spettro di effetti su organismi con risposte differenti ai vari composti presenti

  17. Project LIFE14 ENV/IT/113 - Demonstration of the suitability of dredged remediated sediments for safe and sustainable horticulture production Action B.1: Analysis of sediment eco-toxicity • Microbial toxicity test • Phytotoxicity test • Microcrustacean toxicity test Toxicity threshold: 20% inhibition of the measured endpoints (Persoone et al., 2003)

  18. Sediment ecotoxicity Microbial toxicity: Vibrio fischeri test Microbial toxicity during landfarming Aboatox Oy Inhibition% = 100 - [(TL15/ KF *TL0) * 100] KF = CL15/CL0

  19. Sediment ecotoxicity: Microbial toxicity: Vibrio fischeri test Microbial toxicity: growing media

  20. Sediment ecotoxicity: Phytotoxicity test MicroBioTests Inc. S. Saccharatum (sorghum) L. Sativum (watercress) Inh% = 100 * (C – T)/C S. Alba (mustard)

  21. Sediment ecotoxicity: Phytotoxicity test Phytotoxicity during landfarming

  22. Sediment ecotoxicity: Microcrustacean toxicity: Daphnia magna Microcrustacean toxicity during landfarming PE= 100 * N.I./T N.I. = n° immobilized neonates T = n° total neonates

  23. Eco toxicity after strawberry plant growth

  24. Action C.1: Monitoring and validation of treated sediments Previous results: no phytoremediation sediment toxicity during plant growth

  25. Test ecotossicologici: relazione con la normativa La medianadellaconcentrazioneefficace(EC50) suprocarioti, eucariotiunicellulariedesseriumani di oltre 1200 sostanzechimichehannoeffetticomparabili con le risposte del test dell’inibizionedellabioluminescenza di V. fischeri QuershiAA, Bulich AA, Isenberg DL. 1998. Microtox toxicity test systems— where they stand today. In: Wells PG, Lee K, Blaise C, editors. Microscale testing in aquatic toxicology advances, techniques, and practice. Boca Raton: CRC Press; pp. 185–199.

  26. Conclusions Phytoremediated sediment showed no significant ecological toxicity The main reason for hindrance in the sediment use for horticulture is its origin from a National Priority Interest Polluted site (SIN) Because phytoremediated sediments from a ‘worst scenario’ responded positively, dredged sediments may pass all tests requested by the current legislation for growing media

  27. - The end - Thanks for the attention

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