1 / 4

Colorimetric Estimation of Lipids Using Vanillin-Phosphovanillin Method

This method involves the use of sulfuric acid and vanillin phosphate ester to form a purple complex for lipid estimation. The process uses standard solutions of cholesterol and olive oil to determine lipid content based on optical density readings. By calculating the absorbance of the unknown and standard samples, lipid concentrations can be determined accurately.

mhorn
Download Presentation

Colorimetric Estimation of Lipids Using Vanillin-Phosphovanillin Method

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Estimation of Total Lipids by Colorimetric Method * Principle LIPIDS H2SO4 Carbonium ions + + Vanillin phosphate ester Purple Complex

  2. Materials • Phosphovanillin reagent • Sulfuric acid • Standard solution of cholesterol (1g/100 ml acetone) • Standard solution of olive oil

  3. 60 5 55 10 50 45 15 40 20 35 25 30 15min. 10 min. St. Unk. St. Unk. 20µ unk. 20µ unk. 20µ st. 20µ st. 20µ D.W. Phosphovanillin 10 10 10 10 10 (ml) H2SO4(ml) 0.2 0.2 0.2 0.2 0.2 Mix Well on a vortex mixer Mix Well COOL in water at Room Temperature Incubate at 37ºC in water bath Read the Optical Density at 540 nm

  4. * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard CUn. = A Un. × C St. A St. Samar A. Damiati

More Related