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Organization of the Cell. Cell theory Cells are the basic living units of organization and function All cells come from other cells Work of Schleiden, Schwann, and Virchow contributed to this theory Each cell is a microcosm of life. Biological size and cell diversity.
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Cell theory • Cells are the basic living units of organization and function • All cells come from other cells • Work of Schleiden, Schwann, and Virchow contributed to this theory • Each cell is a microcosm of life
Cell surface area-to-volume ratio • Plasma membrane must be large enough relative to cell volume to regulate passage of materials • Cell size and shape related to function
Microscopes • Light microscope, referred to as compound microscope, used by most students • Two features determine how clearly an object is viewed • Magnification • Resolution • Light microscope has 500 times more resolution than human eye
Special optical mechanisms for light microscopes • Bright-field microscopy • Phase contrast microscopy • Differential-interference-contrast microscopy • Fluorescence microscope, including confocal fluorescence microscope
Electron microscope • Developed in the 1950s • Allows study of the ultrastructure of cells • 10,000 times more resolution than human eye
Types of electron microscope • Transmission electron microscope • Scanning electron microscope
Cell fractionation • Used to determine function of organelles • Cells broken apart and the resulting cell extract spun in a centrifuge • Centrifugal force separates extract • Pellet • Supernatant
Prokaryotic • Bacteria and archaea • DNA not enclosed in a nucleus • Eukaryotic • All other known organisms • Highly organized membrane-enclosed organelles • Cytoplasm • Nucleoplasm
Functions of cell membranes • Divide cell into compartments, allowing for specialized activities • Interacting membranes form endomembrane system • Vesicles transport materials between compartments
The cell nucleus • Contains DNA • Bounded by • Nuclear envelope • Double membrane perforated with nuclear pores • DNA forms chromatin, which is organized into chromosomes • Nucleolus • RNA synthesis and ribosome assembly
Endoplasmic reticulum (ER) • Network of folded internal membranes in the cytosol • Smooth ER • Site of lipid synthesis • Site of detoxifying enzymes • Rough ER • Ribosomes manufacture proteins • Proteins may be moved into the ER lumen
Golgi complex • Cisternae that process, sort, and modify proteins • In animal cells, Golgi complex also manufactures lysosomes • Glycoproteins transported to the cis face • Golgi modifies carbohydrates and lipids and packages into vesicles
Lysosomes break down worn-out cell structures, bacteria, and other substances • Peroxisomes • Involved in lipid metabolism and detoxification • Contain enzymes that produce and degrade hydrogen peroxide
Mitochondria • Sites of aerobic respiration • Organelles enclosed by a double membrane • Place important role in apoptosis • Cristae and matrix contain enzymes for aerobic respiration • Nutrients broken down and energy packaged in ATP • Carbon dioxide and water by-products
Chloroplasts • Plastids that carry out photosynthesis • Inner membrane of chloroplast encloses the stroma • During photosynthesis, chlorophyll traps light energy • Energy converted to chemical energy in ATP
Cytoskeleton • Internal framework made of • Microtubules • Microfilaments • Intermediate filaments • Provides structural support • Involved with transport of materials in the cell
Cilia and flagella • Thin, movable structures that project from cell surface • Function in movement • Microtubles anchored in cell by basal body
Glycocalyx, cell coat formed by polysaccarides extending from plasma membrane • Many animal cells also surrounded by an extracellular matrix (ECM) • Most bacteria, fungi, and plant cell walls made of carbohydrates