Lecture 9. Assignment 3 Yeast complementation analysis Discussion of Article 6. The article for the final examination is posted. Remember I do not discuss this article with you. Final Examination Date and Time Wednesday Dec. 17, 2008 7:00 PM. Assignment 3.
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Dec. 17, 2008
Example of arginine biosynthesis. There are several enzymes involved in this process, and we want to generate mutants in all of them. So, we mutagenize yeast and look for auxotrophs of arginine.
Generate several mutants and cross them all with each other
Can discover a series of complementation groups.
Mutants 1 and 4 can complement each other. They are therefore in different genes. Mutants 1 and 2 do not complement each other. They are in the same gene and in the same complementation group.
B. Haploid cells forming shmoos and zygotes
C. Zygote budding off diploid
D. Diploid budding
E. Diploid forming asci (spore-containing sacs)
& freed haploid spores
This week you
will cross your
the parental to
the swi5 tester strain on a
YEPD rich plate.
Next week you will put the
mated cells on diploid selection
plates; score red/white a week later.