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WedNesday April 8 th 2014. QU: What does it mean to recombine? **Chapter 13 Test April 17 th 2014** OBJ: Bio-Technology “The life of an eyelash is about 5 months.”. DNA TECHNOLOGY: Biotechnology. Recombinant DNA Process. Recombinant DNA.

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wednesday april 8 th 2014
WedNesday April 8th 2014
  • QU: What does it mean to recombine?

**Chapter 13 Test April 17th 2014**

  • OBJ: Bio-Technology

“The life of an eyelash is about 5 months.”

dna technology biotechnology
DNA TECHNOLOGY:Biotechnology

Recombinant DNA Process

recombinant dna
Recombinant DNA
  • Some of the most important techniques used in biotechnology involve making recombinant DNA molecules
recombinant dna1
Recombinant DNA
  • Recombinant DNA molecules are pieces of DNA that have been combined from pieces taken from more than one source of DNA
    • Often one of the sources is a Plasmid, the other is contains a gene we want
plasmids
Plasmids
  • Small circular DNA molecules that can be in cells.
    • Often contain genes for resistance to antibiotics.
      • They are also used for human benefit.
restriction enzymes
Restriction Enzymes
  • Possible because of special enzymes that cut DNA: called RESTRICTION ENZYMES
dna scissors intro to restriction enzymes
DNA Scissors: Intro to Restriction Enzymes
  • Restriction enzymes recognize and bind to specific DNA sequences and cut the DNA at or near this site.
restriction sites
Restriction Sites
  • These recognition cleave sites are called restriction sites.
  • Each different restriction enzyme has its own type of site.
restriction enzymes1
Restriction enzymes
  • Can distinguish between the DNA normally present in the cell and foreign DNA, such as infecting bacteria virus DNA
  • The enzymes defend the cell from invasion by cutting the foreign DNA into pieces so it doesn’t work.
restriction enzymes2
Restriction Enzymes
  • Restriction enzymes appear to be made exclusively by prokaryotes. (bacteria)
    • So bacteria is vital to DNA Technology because it makes the restriction enzymes.
thursday april 10 th 2014
Thursday April 10th 2014
  • QU: What are restriction enzymes?
  • OBJ: Restriction Enzyme Lab, Notes

“Rats can swim for half a mile (0.8 km) without rest, and can tread water for three straight days.”

**Chapter 13 Test April 17th 2014**

recombinant dna2
Recombinant DNA
  • Identify the desired gene
  • Cut the gene out of the DNA Strand with restriction enzymes
  • Cut the plasmid with the same restriction enzymes and added the desired gene to the plasmid by matching the sticky ends
  • Place plasmid in the bacteria
    • Allow the bacteria to replicate and clone the gene
      • This replicates the desired gene
      • Protein synthesis creates the desired protein
genetic modification
Genetic Modification
  • Genetically Modified Organism (GMOs)
    • Any organism that has acquired genes by artificial means.
    • Replaces traditional plant breeding
    • The goal is to make it bigger, better, more desirable organisms
how gmos are created
How GMOs are created?
  • Animals
    • Injecting a gene into a fertilized egg and then returning that egg to a uterus where it can develop
  • Plants
    • Introducing recombinant plasmid into a plant cell and letting it grow into a plant.
friday april 11 th 2014
Friday April 11th 2014
  • QU: Describe Recombinant DNA?

Journal Due Today

Chapter 13 Test April 17th

  • OBJ: Recombinant DNA Lab

“The Giant Burrowing Frog doesn't croak. It hoots like an owl.”

monday april 14 th 2014
Monday April 14th 2014
  • QU: Why is it important to be able to replicate our plasmid?

*Chapter 13 Test April 17th*

  • OBJ: DNA Fingerprinting Notes and Activity

“The world's smallest rodent is Pygmy Jerboa - measuring only a couple of inches in length - and can run as fast as a horse.”

dna fingerprinting
DNA Fingerprinting
  • How is DNA Fingerprinting done?
  • Scientists use the variable regions in our DNA to generate a DNA profile of an individual, using samples from blood, bone, hair, and other body tissues and products.
slide22
In criminal cases, this generally involves obtaining samples from crime-scene evidence and a suspect, extracting the DNA, and analyzing it for the presence of a set of specific DNA regions (markers).
  • Forensic scientists compare these DNA profiles to determine whether the suspect's sample matches the evidence sample.
slide23
To get the DNA fingerprint:
  • All of the DNA is broken into pieces at certain locations.
    • Done by restriction enzymes
  • The DNA pieces are placed at the top of a special gel held in a frame.
    • An electric current is applied to the gel. The current separates the DNA into bands of identical pieces. This process is called electrophoresis.
    • This electric current pulls the negatively charged DNA to the positive end of the gel.
last notes on dna fingerprinting
Last Notes on DNA Fingerprinting
  • DNA Fingerprinting moves DNA by applying an electric current during electrophoresis.
    • This electric current pulls the negatively charged DNA to the positive end of the gel.
dna fingerprinting1
DNA Fingerprinting
  • The band pattern is transferred to a nylon membrane containing a radioactive chemical. The chemical makes the bands show up clearly.
why it is done
Why It Is Done

DNA fingerprinting is done to:

  • Determinewho a person's parents or siblings are.
    • This test also may be used to identify the parents of babies who were switched at birth.
  • Solve crimes (forensic science).
    • Blood, skin, or other tissue left at the scene of a crime can be analyzed to help prove whether the suspect was or was not present at the crime scene.
  • Identify a body.
    • This is useful if the body is badly decomposed or if only body parts are available, such as following a natural disaster or battle.
wednesday april 16 th 2014
WedNesday April 16th 2014
  • QU: Describe how DNA fingerprinting works.

*Chapter 13 Test Tomorrow*

Chapter 13 Review Due tomorrow

  • OBJ: DNA Fingerprinting, Review for Ch 13 Test

“The normal energy used by our brain is 0.1 calories per minute, and could go up to 1.5 during activities such as puzzle-solving.”

thursday april 17 th 2014
Thursday April 17th 2014
  • QU: How did you study for this test?

*Turn your chapter review into the HW bin*

  • OBJ: DNA Technology Test, Organize Binder

“The normal energy used by our brain is 0.1 calories per minute, and could go up to 1.5 during activities such as puzzle-solving.”

binder organization ch 13
Binder Organization: Ch 13
  • DNA Technology Hand Written Notes
  • Restriction Enzyme Lab
  • Recombinant DNA Lab
  • Sub work (Book Work)
  • Quiz #1 DNA Technology
  • DNA Fingerprinting worksheet
  • DNA Technology
  • Chapter 13 Review
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