Learning Objectives • Learn how the rate of an enzyme controlled reaction is measured. • Learn how temperature affects the rate of an enzyme controlled reaction. • Learn how pH affects the rate of an enzyme controlled reaction. • Learn how substrate concentration affects the rate of reaction.
How to measure the progress of enzyme-catalysed reactions... • Generally, one of two ‘outcomes’ are measured over time: - the formation of the products of the reaction. - the disappearance of the substrate.
Draw and explain the following graphs... Mass of starch Volume of oxygen produced Time Time Enzyme-catalysed reaction in which oxygen is formed due to the action of catalase on hydrogen peroxide. Enzyme-catalysed reaction in which starch disappears due to the reaction of amylase.
The Effect of Temperature on the Rate of an Enzyme-Controlled Reaction
The Effect Of Changing Substrate Concentration The effect of changing the amount of substrate on the rate of an enzyme-controlled reaction. As the amount of substrate increases from 0 to B, the rate of reaction increases. As the amount of substrate increases from B to C, the rate of reaction does not increase any further.