University of Pennsylvania . Mesothelioma Collections September 2010 through September 2011. Work-Flow. Collaborative Effort. Patient. The Process.
September 2010 through September 2011
Melissa Culligan consents the patient.
Surgery date isforwarded to TTAB
Dr. Friedberg performs the surgery
OR nurse pages TTAB tech when tissue is available
TTAB tech picks up blood & tissue & processes them for cryopreservation
What happens to the tissue?
Formalin-fixed-paraffin- embedded (FFPE)
15 Plasma CPT aliquots
15 Plasma EDTA aliquots
10 Serum aliquots
5 Packed RBCs
2 PBMC aliquots
3 Whole bloods
4 Buffy coat aliquots
5 Urine aliquotsThe Collection…
Histologic confirmation & classification by Dr. Feldman.
Technician enters data in NMVB database.
Sept. 2010 through Sept 2011
19 Subjects consented for tissue and fluid collections.
Sept. 2009 through Sept. 2010
1 Retrospective cases have been identified and retrieved from archives.
These are now available to NMVB as paraffin blocks.
138 Fresh-frozen tumor samples
100 OCT blocks of tumor
138 Paraffin embedded blocks
Normal Adjacent Tissue:
4 Fresh-frozen tissue samples
2 Paraffin embedded blocks
475 Plasma aliquots
102 Serum aliquots
70 Packed RBCs
22 PBMC aliquots
42 Whole bloods
61 Buffy coat aliquots
388 Pleural Fluid aliquots
24 Cell Pellets
20 Supernatant aliquots
62 Urine aliquots
We did whatever it took, as long as we had dedicated bench space, to do our job.
Four -80 freezers,
Two -140 freezers,
One -20 Freezer,
One liquid nitrogen vapor-phase storage tank, and
One 4 degree refrigerator.
All are monitored 24/7 with staff providing on-call services in the event of a malfunction.
Fully equipped for biospecimen processing:
Plenty of room for growth in the years to come…
A Vectra spectral imaging microscope is a recent acquisition at TTAB.
We seek to integrate this technology into all projects.
Nancy Whelan, Dr. Michael Becich, & Dr. Waqas Amin