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University of Pennsylvania

University of Pennsylvania . Mesothelioma Collections September 2010 through September 2011. Work-Flow. Collaborative Effort. Patient. The Process.

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University of Pennsylvania

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  1. University of Pennsylvania Mesothelioma Collections September 2010 through September 2011

  2. Work-Flow

  3. Collaborative Effort Patient

  4. The Process • Melissa Culligan, RN, CCRC for the thoracic surgical team, approaches and consents patients for biospecimen collection. Once the patient consents, Melissa contacts the TTAB technician with the patient’s information. • On the day-of-surgery, the TTAB technician tracks the progression of the surgical procedure using NaviCare, the hospital’s patient tracking software. • One hour after incision occurs the technician accesses the OR to collect blood and urine, if available, and returns to the TTAB bench located within Surgical Pathology, which is less than 2 minutes away. • While processing the fluids at the TTAB bench, the Tech will receive a page that tissue is available for pick-up. Melissa Culligan consents the patient. Surgery date isforwarded to TTAB 1 2 Dr. Friedberg performs the surgery 3 OR nurse pages TTAB tech when tissue is available 4 TTAB tech picks up blood & tissue & processes them for cryopreservation

  5. The Collection

  6. The Collection What happens to the tissue? Snap-frozen OCT embedded Formalin-fixed-paraffin- embedded (FFPE)

  7. Typical collection yields 50 cc of blood & 75 cc of urine,… which is then processed into: 15 Plasma CPT aliquots 15 Plasma EDTA aliquots 10 Serum aliquots 5 Packed RBCs 2 PBMC aliquots 3 Whole bloods 4 Buffy coat aliquots 5 Urine aliquots The Collection…

  8. QA Histologic confirmation & classification by Dr. Feldman. Technician enters data in NMVB database.

  9. Prospective Collections Sept. 2010 through Sept 2011 19 Subjects consented for tissue and fluid collections. • 16 Pleurectomies(7 with associated biopsies) • 3 Biopsies(no other procedure performed) • 2 Thoracenteses(de-identified; no other procedure performed)

  10. Sept 2009 – Sept 2010 23 Consented Cases & 2 De-Identified Case: 18 Pleurectomies 5 Biopsies 2 Thoracenteses (de-ID) Sept 2009 – Sept 2010 19 Consented Cases & 1 De-Identified Case: 16 Pleurectomies 3 Biopsies 1 Thoracenteses (de-ID) Looking back in comparison…

  11. Retrospective Collections Sept. 2009 through Sept. 2010 1 Retrospective cases have been identified and retrieved from archives. These are now available to NMVB as paraffin blocks.

  12. What’s this mean to NMVB as a resource?

  13. Prospectives’ Specimen Yield, in Total… Tumor Tissue: 138 Fresh-frozen tumor samples 100 OCT blocks of tumor 138 Paraffin embedded blocks Normal Adjacent Tissue: 4 Fresh-frozen tissue samples 2 Paraffin embedded blocks Fluid Biospecimens: 475 Plasma aliquots 102 Serum aliquots 70 Packed RBCs 22 PBMC aliquots 42 Whole bloods 61 Buffy coat aliquots 388 Pleural Fluid aliquots 24 Cell Pellets 20 Supernatant aliquots 62 Urine aliquots

  14. TTAB, the Facility… • Occupied for just 3 years… TTAB has operated out of temporary offices, closets, server rooms, etc. for a year & a half prior to moving into the new facility. We did whatever it took, as long as we had dedicated bench space, to do our job.

  15. The Freezer Room Four -80 freezers, Two -140 freezers, One -20 Freezer, One liquid nitrogen vapor-phase storage tank, and One 4 degree refrigerator. All are monitored 24/7 with staff providing on-call services in the event of a malfunction.

  16. The Lab Fully equipped for biospecimen processing: • embedding machine • 2 microtomes • 2 centrifuges • slide staining station • microscope

  17. The Lab Plenty of room for growth in the years to come…

  18. The Imaging Room A Vectra spectral imaging microscope is a recent acquisition at TTAB. We seek to integrate this technology into all projects.

  19. Special thanks to… Nancy Whelan, Dr. Michael Becich, & Dr. Waqas Amin

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