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University of Pennsylvania . Mesothelioma Collections September 2010 through September 2011. Work-Flow. Collaborative Effort. Patient. The Process.

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university of pennsylvania

University of Pennsylvania

Mesothelioma Collections

September 2010 through September 2011

the process
The Process
  • Melissa Culligan, RN, CCRC for the thoracic surgical team, approaches and consents patients for biospecimen collection. Once the patient consents, Melissa contacts the TTAB technician with the patient’s information.
  • On the day-of-surgery, the TTAB technician tracks the progression of the surgical procedure using NaviCare, the hospital’s patient tracking software.
  • One hour after incision occurs the technician accesses the OR to collect blood and urine, if available, and returns to the TTAB bench located within Surgical Pathology, which is less than 2 minutes away.
  • While processing the fluids at the TTAB bench, the Tech will receive a page that tissue is available for pick-up.

Melissa Culligan consents the patient.

Surgery date isforwarded to TTAB



Dr. Friedberg performs the surgery


OR nurse pages TTAB tech when tissue is available


TTAB tech picks up blood & tissue & processes them for cryopreservation

the collection1
The Collection

What happens to the tissue?


OCT embedded

Formalin-fixed-paraffin- embedded (FFPE)

the collection2
Typical collection yields 50 cc of blood & 75 cc of urine,… which is then processed into:

15 Plasma CPT aliquots

15 Plasma EDTA aliquots

10 Serum aliquots

5 Packed RBCs

2 PBMC aliquots

3 Whole bloods

4 Buffy coat aliquots

5 Urine aliquots

The Collection…

Histologic confirmation & classification by Dr. Feldman.

Technician enters data in NMVB database.

prospective collections
Prospective Collections

Sept. 2010 through Sept 2011

19 Subjects consented for tissue and fluid collections.

  • 16 Pleurectomies(7 with associated biopsies)
  • 3 Biopsies(no other procedure performed)
  • 2 Thoracenteses(de-identified; no other procedure performed)
looking back in comparison
Sept 2009 – Sept 2010

23 Consented Cases &

2 De-Identified Case:

18 Pleurectomies

5 Biopsies

2 Thoracenteses (de-ID)

Sept 2009 – Sept 2010

19 Consented Cases


1 De-Identified Case:

16 Pleurectomies

3 Biopsies

1 Thoracenteses (de-ID)

Looking back in comparison…
retrospective collections
Retrospective Collections

Sept. 2009 through Sept. 2010

1 Retrospective cases have been identified and retrieved from archives.

These are now available to NMVB as paraffin blocks.

prospectives specimen yield in total
Prospectives’ Specimen Yield, in Total…

Tumor Tissue:

138 Fresh-frozen tumor samples

100 OCT blocks of tumor

138 Paraffin embedded blocks

Normal Adjacent Tissue:

4 Fresh-frozen tissue samples

2 Paraffin embedded blocks

Fluid Biospecimens:

475 Plasma aliquots

102 Serum aliquots

70 Packed RBCs

22 PBMC aliquots

42 Whole bloods

61 Buffy coat aliquots

388 Pleural Fluid aliquots

24 Cell Pellets

20 Supernatant aliquots

62 Urine aliquots

ttab the facility
TTAB, the Facility…
  • Occupied for just 3 years… TTAB has operated out of temporary offices, closets, server rooms, etc. for a year & a half prior to moving into the new facility.

We did whatever it took, as long as we had dedicated bench space, to do our job.

the freezer room
The Freezer Room

Four -80 freezers,

Two -140 freezers,

One -20 Freezer,

One liquid nitrogen vapor-phase storage tank, and

One 4 degree refrigerator.

All are monitored 24/7 with staff providing on-call services in the event of a malfunction.

the lab
The Lab

Fully equipped for biospecimen processing:

  • embedding machine
  • 2 microtomes
  • 2 centrifuges
  • slide staining station
  • microscope
the lab1
The Lab

Plenty of room for growth in the years to come…

the imaging room
The Imaging Room

A Vectra spectral imaging microscope is a recent acquisition at TTAB.

We seek to integrate this technology into all projects.

special thanks to
Special thanks to…

Nancy Whelan, Dr. Michael Becich, & Dr. Waqas Amin