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lectin affinity enrichment (WGA)

Control (CTL). +TGF- β. BRI-JM01/ NMuMG cells. lectin affinity enrichment (WGA). trypsin. trypsin. nano-LC-MS analysis (Q-TOF). x 3 biological repetitions. (3 MS replicates/sample). Quantitation of peptide elution profiles. Quantitation of peptide elution profiles.

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lectin affinity enrichment (WGA)

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  1. Control (CTL) +TGF-β BRI-JM01/ NMuMG cells lectin affinity enrichment (WGA) trypsin trypsin nano-LC-MS analysis (Q-TOF) x 3 biological repetitions (3 MS replicates/sample) Quantitation of peptide elution profiles Quantitation of peptide elution profiles Matching of peptides across data files Determination of differentially expressed peptide peaks MatchRx software Identification of differentially-expressed peptides by MSMS (1) include list (Q-TOF) (2) auto-MSMS (LTQ/Q-TOF) Determination of differentially expressed PROTEINS

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