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INTRODUCTION

A. B. DECREASED PERIPHERAL BLOOD BDCA1+ AND BDCA3+ DENDRITIC CELLS IN RHEUMATOID ARTHRITIS AND OSTEOARTHRITIS.

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INTRODUCTION

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  1. A B DECREASED PERIPHERAL BLOOD BDCA1+ AND BDCA3+ DENDRITIC CELLS IN RHEUMATOID ARTHRITIS AND OSTEOARTHRITIS M VILLARROEL1, G ZALDIVAR1, MA SÁNCHEZ1, A HERNÁNDEZ1, JDD GARCÍA2, MJ LEÓN3, A SÁNCHEZ-ATRIO3, A PÉREZ-GÓMEZ3, E CUENDE3, A LÓPEZ3, F ALBARRAN3, J MONSERRAT1, A PRIETO1, E REYES1, M ALVAREZ-MON1,3 • Laboratory of Immune System Diseases and Oncology. R&D CNB-CSIC associated unit. Department of Medicine, Alcalá de Henares, Spain. • Department of Internal Medicine. “Principe de Asturias” University Hospital, Alcalá de Henares, Spain. • Immune System Diseases and Oncology Service. “Principe de Asturias” University Hospital, Alcalá de Henares, Spain. INTRODUCTION Circulating Peripheral Blood Dendritic Cells (CPB-DCs) comprise different subsets that are involved into inflammatory response of patients suffering autoimmune and degenerative chronic diseases. AIM The aim of this study is to compare the distribution of CPB-DC subsets in patients with rheumatoid arthritis (RA), osteoarthritis (OA) and healthy controls (HC). Figure 2. Distribution of mDC1 and mDC2 subsets in CPB-DCs. Percentage distribution. A. mDC1. anti BDCA1+ B. mDC2. anti BDCA3+ Numbers represent the significant values of U Mann Whitney test. Blue box differences between OA and HC, orange box differences between RA and HC. RA. Rheumatoid arthritis. OA osteoarthritis. HC. Healthy controls. We found similar percentages of both mDC1 and mDC2 subsets between RA and OA patients. The percentage of CD19-CD14-EMA-BDCA2+ DC subset did not differ in all three groups studied (Fig 3). Figure 3. Distribution of pDC subset in CPB-DCs Percentage distribution. RA. Rheumatoid arthritis. OA osteoarthritis. HC. Healthy controls CONCLUSION We found a significant decrease in mDC subset in RA and OA patients compare to HC. This data also could be explained partially by the chronic inflammatory response in both pathologies. The decreased percentage of mDC subset found in these patients may reflect either increased apoptosis of these cells or alternatively to enhanced migration to sites of inflammation. • Figure 1. Flow cytometric analysis of CPB-DCs. • The right contour plots showed the different CPB-DCs subsets, assessed in the CD19-CD14-EMA- population. • Sample stain with cocktail control. • Sample stain with DBCA cocktail. • Abreviations: FITC, fluorescein isothiocyanate; PE, phycoerithrin; PE-Cy5.5, phycoerithrin-cyanin 5.5; APC, allophyco-cyanin. EMA. Ethidium Bromide Monoazide.

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