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Protein Immunodetection to Determine the Effects of Maternal Infection in the Perinatal Rat Cerebellum

Background. . Maternal InfectionBacterial VaginosisUrinary Tract InfectionChorioamnionitis. . Intrauterine InfectionMaternal cytokine activation. . Fetal Inflammatory Response Cytokine activation. . Neurological DamagePeriventricular LeukomalaciaCerebral PalsySchizophreniaAutism. Ba

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Protein Immunodetection to Determine the Effects of Maternal Infection in the Perinatal Rat Cerebellum

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    1. Protein Immunodetection to Determine the Effects of Maternal Infection in the Perinatal Rat Cerebellum Sai Sruthi Veerisetty

    2. Background

    3. Background Cerebellum

    4. Results from Last Summer

    5. Results from Last Summer

    6. Conclusions from Last Summer Colocalization studies showed that saline treated rats had more Calbindin and TNF-a immuno reactivity than the E. coli treated rats

    7. Purpose To analyze Calbindin and TNF-a protein levels in the Cerebellum to see if the immunohistochemistry staining correlated with protein expression To analyze the number of Purkinje cells in the Purkinje cell layer of the vermis

    8. Methods Pregnant rats were given .1 cc of saline or 106 CFU E. coli bacteria in the uterine horn Pups were sacrificed on PN8 and PN16 Brains were dissected and held at -80C until used Whole cerebellums were used

    9. Methods- Western Blot Prepare sample by sonicating tissue Run gel electrophoresis Transfer gel to a membrane Incubate membrane with 1 and 2 antibody Expose and develop membrane

    10. Results

    11. Results

    12. Results and still counting!

    13. Future Studies If there is a decrease in Calbindin, then we will conduct behavioral studies to see if the decrease affects motor coordination in the rat pups If there is not a decrease in Calbindin, then we will have to look to see if there is a decrease in the amount of receptors mRNA studies can be conducted to see if Calbindin is not being transcribed

    14. Acknowledgements Dr. William Bennett Kedra Wallace Dr. Javier Miguel-Hidalgo Dr. Dorata Maciag Dr. Ian Paul CPN Neuroscience Scholars Summer Program

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