1 / 9

PNA-mediated Whiplash PCR

PNA-mediated Whiplash PCR. J. A. Rose et al. DNA7 poster Summarized by Shin, Soo-Yong 2001. 8. 8. Abstract. Whiplash PCR (WPCR) Recursive, self-directed polymerase extension of a mixture of DNA hairpins. Problems : backhybridization

manelin-inara
Download Presentation

PNA-mediated Whiplash PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PNA-mediated Whiplash PCR J. A. Rose et al. DNA7 poster Summarized by Shin, Soo-Yong 2001. 8. 8

  2. Abstract • Whiplash PCR (WPCR) • Recursive, self-directed polymerase extension of a mixture of DNA hairpins. • Problems : backhybridization • In this paper, WPCR is modeled by a statistical thermodynamic model & Markov chain. • To prevent backhybridization, PNA2/DNA triplex formation (P-WPCR) will be proposed. (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  3. WPCR • Computational steps are implemented by the template-directed, recursive polymerase extension of a mixture of DNA hairpins. (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  4. WPCR • In the original protocol, computation begins with a reduction in reaction temperature. • A computational transition occurs by the polymerase extension of hybridization head. • Stop sequence is implemented by a length 3 poly0A sequence (absence of free dTTP) • After polymerization, hairpins are thermally denatured. (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  5. Drawbacks of WPCR • A tendency for extended molecules to participate in backhybridication More stable Less stable (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  6. Statistical model • Assume encounters/strand • More study is needed. (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  7. PNA mediated WPCR • WPCR architecture may be redesigned to enable the specific inhibition of backhybridized structures by targeted PNA2/DNA triple formation. • Polyamine nucleic acid (PNA) bind to complementary ssDNA with extremely high affinity • Under appropriate reaction condition, rapid, irreversible formation of the triplex structure occurs even if the target DNA single strand is involved in hybridization. (strand invasion) • Triplex formation : P-loop (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  8. PNA mediated WPCR • The sequence TTTTCTTCTT • ssDNA target sequence of the highly efficient cationic bis-PNA molecules. (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

  9. PNA mediated WPCR (C) 2001, SNU Biointelligence Lab, http://bi.snu.ac.kr/

More Related