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제 35 회 LAB MEETING

제 35 회 LAB MEETING. Chromosome Research 10: 55-61, 2002 . DIRECT EVIDENCE FOR THE Homo-Pan clade. 2002. 4, 8. 석사 1 년차 최 진. 최근까지 많은 DNA date 는 그들의 가까운 molecular similarity 때문에 Home, Pan, Gorilla 사이의 관계를 풀지 못하고 있다 . 여기서 우리는 다른 실험적 방법을 사용하여 Homo-pan clade 의 직접적인 증거를 제시한다.

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제 35 회 LAB MEETING

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  1. 제 35회 LAB MEETING Chromosome Research 10: 55-61, 2002. DIRECT EVIDENCE FOR THE Homo-Pan clade 2002. 4, 8 석사 1년차 최 진

  2. 최근까지 많은 DNA date는 그들의 가까운 molecular similarity 때문에 Home, Pan, Gorilla 사이의 관계를 풀지 못하고 있다. • 여기서 우리는 다른 실험적 방법을 사용하여 Homo-pan clade 의 직접적인 증거를 제시한다.

  3. <INTRODUCTION> “TRICHOTOMY PROBLEM” Homo-pan clade The trichotomy

  4. * Such mismatches can be due to two factors • 한정된 수의 nucleotides를 sequencing하기 때문. • Genetic polymorphism (in ancestral species).

  5. <MATERIALS AND METHODS> Chromosome preparation Human (Homo sapiens) Chimpanzee (Pan troglodytes) Pygmy chimpanzee or Bonobo (Pan paniscus) Western lowland gorilla (Gorilla gorilla gorilla) Eastern lowland gorilla (Gorilla gorilla graueri) Sumatran orngutan (Pongo pygmaeus abelii) Bornean orangutan (Pongo pygmaeus pygmaeus) White-handed gibbon (Hylobates lar) Pig-tailed macaque (Macaca nemestrina) Proboscis monkey (Nasalis larvatus) Fluorescence in-situ hybridization(FISH) Marker for the pseudoautosomal gene SHOX The PAC and BAC clones derived from the Y-chromosomal contig. Fluorescence microscopy and imaging Zeiss Axiophot epifluorescence microscope : red, green, blue

  6. <Results and discussion> To be continued next page

  7. <Conclusion> • Y chromosome위로 1번 chromosome의 약 100kb의 작은 DNA조각의 Transposition은 Human, chimpanzee, bonobo의 공동조상에서 일어났음이 틀림없다고 보여진다. • 그러므로, 이러한 transposition은 Homo-Pan clade의 명확하고 확실한 증거를 제시해준다. • 이러한 결과는 또한 인류의 진화적인 역사를 푸는데 Y chromosome 의 연구의 강력한 힘을 다시 한번 강조시켜 주었다.

  8. <References> • Molecular Phylogeny of the Hominoids : Inferences from Multiple • Independent DNA Sequence Data Sets. Maryellen Ruvolo. Mol. Biol.Evol.1997 • 2. Recent African origin of modern humans revealed by complete sequences of homioid mitochondrial DNAs. Satoshi Horai. Proc. Natl.Acad. Sci. USA. 1995 • 3. Toward a Phylogenetic Classification of Primates Based on DNA Evidence Complemented by Fossil Evidence. Morris Goodman. Mol.Phyl.Evol.1998 • 4.Evolution of a HOXB6 intergenic region within the great apes and humans. Amos Deinard. Journal of Human Evolution1999

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