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Microbiota Evolution in E. coli and E. faecalis Groups

Study on evolution of E. coli and E. faecalis in monoxenic and dixenic groups using RT-qPCR quantification of bacterial species. Monitoring of bacterial groups at different time points after inoculation. Comparison of human and farm animal microbiota.

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Microbiota Evolution in E. coli and E. faecalis Groups

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  1. A B C Figure S4: Evolution of E. coli and E. faecalis in monoxenic and dixenic groups. Bacterial groups were quantified by RT-qPCR with the species-specific 16S rRNA gene-targeted primers described by (Furet et al., 2009). (A) Monitoring of E. coli CEC15 in the Monocoli-group, 3, 7 and 21 days after inoculation (n = 8 per group). (B) Monitoring of E. faecalis CEF15 in the Monofaecalis-group, 3, 7 and 21 days after transfer (n = 8 per group). (C) Monitoring of E. coli CEC15 and E. faecalis CEF15 in the Dicolifaecalis-group, 3, 7 and 21 days after transfer (n = 8 per group). Furet J, Firmesse O, Gourmelon M, Bridonneau C, Tap J, Mondot S et al. (2009). Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR. FEMS microbiology ecology 68:351-362.

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