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Techniques in Polypeptide Analysis: A Comprehensive Guide

Explore the methods for separating, analyzing, and synthesizing polypeptides, including ion exchange chromatography, cleavage of disulfide bridges, Edman degradation, and solid-phase peptide synthesis. This practical guide covers various techniques from Ninhydrin tests to carboxypeptidases cleavage, providing insights for researchers and students.

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Techniques in Polypeptide Analysis: A Comprehensive Guide

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  1. pI = (pk1 + pk2)/2, the pH where the molecule has a net charge of 0

  2. Ninhydrin Test

  3. Ion Exchange Chromatography

  4. Step 1. Separation of Polypeptide Chains dissociated by exposure to pH extremes, 8 M urea, 6 M guanidinium hydrochloride, or high salt cencentrations

  5. Cleavage of disulfide bridges

  6. Edman degradation phenylisothiocyanate 50 cycles of reaction can be accomplished on 50 pmol (about 0.1 mg)

  7. Carboxypeptidases cleave amino acid residues from the C-termini

  8. Cyanogen bromide

  9. Fmoc solid phase peptide synthesis: A Practical Approach Edited by Weng C. Chan and Peter D. White OXFORD UNIVERSITYPRESS 2000

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