1 / 2

Genetic Engineering Techniques: Molecular Cloning Strategies and Applications

Explore various restriction enzymes like SpeI, NotI, NheI, and their uses in gene manipulation. Learn about pGEM vectors, gene expression, and plasmid isolation methods in molecular biology research.

macaria-roseanne
Download Presentation

Genetic Engineering Techniques: Molecular Cloning Strategies and Applications

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SpeI NotI NotI NheI SpeI pGEM-PlacZ ilvIH SpeI NheI+SpeI pGEM-ilvIH NheI SpeI ilvC SpeI NheI+SpeI pGEM-ilvC NheI SpeI ilvD SpeI NheI+SpeI pGEM-ilvD NheI SpeI kivd SpeI NheI+SpeI pGEM-kivd NheI SpeI adh SpeI NheI+SpeI pGEM-adh SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) NotI NotI IlvIH IlvC IlvD kivd adh pGEM-PlacZ-ilvIH-ilvC-ilvD-kivd-adh NotI+Klenow ScaI pBR322 SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (ScaI/NotI) (ScaI/NotI) IlvIH IlvC IlvD kivd adh pBR-iBO Supplementary Fig. 1.

  2. A PstI PstI PstI 4.2 kb adc ldhA PstI PstI ldhAadc aac(3)IV 6.0 kb B Apr adc [Probe] ldhAadc ldhA adc ldhA ldhA 6.0 kb 4.2 kb Supplementary Fig. 2.

More Related