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IlvIH

Spe I. Not I. Not I. Nhe I. Spe I. pGEM-P lacZ. ilvIH. Spe I. Nhe I+ Spe I. pGEM-ilvIH. Nhe I. Spe I. ilvC. Spe I. Nhe I+ Spe I. pGEM-ilvC. Nhe I. Spe I. ilvD. Spe I. Nhe I+ Spe I. pGEM-ilvD. Nhe I. Spe I. kivd. Spe I. Nhe I+ Spe I. pGEM-kivd. Nhe I. Spe I. adh.

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IlvIH

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  1. SpeI NotI NotI NheI SpeI pGEM-PlacZ ilvIH SpeI NheI+SpeI pGEM-ilvIH NheI SpeI ilvC SpeI NheI+SpeI pGEM-ilvC NheI SpeI ilvD SpeI NheI+SpeI pGEM-ilvD NheI SpeI kivd SpeI NheI+SpeI pGEM-kivd NheI SpeI adh SpeI NheI+SpeI pGEM-adh SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) NotI NotI IlvIH IlvC IlvD kivd adh pGEM-PlacZ-ilvIH-ilvC-ilvD-kivd-adh NotI+Klenow ScaI pBR322 SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (ScaI/NotI) (ScaI/NotI) IlvIH IlvC IlvD kivd adh pBR-iBO Supplementary Fig. 1.

  2. A PstI PstI PstI 4.2 kb adc ldhA PstI PstI ldhAadc aac(3)IV 6.0 kb B Apr adc [Probe] ldhAadc ldhA adc ldhA ldhA 6.0 kb 4.2 kb Supplementary Fig. 2.

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