1 / 15

Neisseria gonorrhoeae and Chlamydia trachomatis co-

Neisseria gonorrhoeae and Chlamydia trachomatis co- Infection: More than just a Coincidence 淋病奈瑟菌与沙眼衣原体共感染：不只是巧合.

mab
Download Presentation

Neisseria gonorrhoeae and Chlamydia trachomatis co-

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Neisseria gonorrhoeae and Chlamydia trachomatis co- Infection: More than just a Coincidence 淋病奈瑟菌与沙眼衣原体共感染：不只是巧合 Sunita Gulati1, Bo Zheng1, Sanjay Ram1, George W. Reed2, Guillermo E. Madico3, Xianbao He3, Robin R. Ingalls3, Christine Chaisson4, Wenjing Le5, Xiaohong Su5 and Peter A. Rice1 1Division of Infect. Diseases and Immunol. and 2Preventive and Behavioral Medicine, Department of Medicine, University of Massachusetts School of Medicine, Worcester MA 01605 3Section of Infectious Diseases, Department of Medicine, Boston University Medical Center, Boston, MA 02118 4Data Coordinating Center, Boston University School of Public Health, Boston, MA 02118 5Nanjing STD Clinic, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, PRP

  2. Study Design • Two epidemiologic studies of Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (Ct) transmission in heterosexual partners:淋病奈瑟菌和沙眼衣原体异性性伴间传播的流行病学研究 • 1) Lin, J-s et al JID, 1998: Conducted in Boston • 2) Ongoing: Conducted in Nanjing • Study design enhances likelihood that transmission occurs from men to women.研究设计旨在保证由男向女的病原体传播方向 • 1) Men are eligible if they have >2 female sex-partners in the 30 days • prior to the onset of their symptoms: men are eligible and enrolled if • they have a Gram’s stain positive for Gram-negative intracellular • diplococci (GNIDs): • Enrolled men may also have Ct infection: • 59/191 (31% co-infection); Boston • 11/46 (24% co-infection); Nanjing • 2) Women are eligible/enrolled if they have been sex-partners of men 2 • weeks before to 2 weeks after male symptoms developed and have • had no other sex-partners; in Nanjing, >95% women are married

  3. Comparison of Gonococcal Genotypes Between Sex-Partners性伴间感染淋球菌的基因型比较-确定传播关系 * *No gonococcal infection transmitted to the female

  4. Chlamydial Genotypes between Ng exposed /infected Sex-Partners AND Pre-Existing Chlamydial Genotypes in Women 暴露并感染淋球菌的性伴 和以前已感染沙眼衣原体女性的沙眼衣原体的基因分型 * *Uninfected with Chlamydia trachomatis (Ct)

  5. Transmission of N. gonorrhoeae (Ng) Infection to Women with and without Pre-Existing C. trachomatis (Ct) infection 女性淋球菌接触者的沙眼衣原体感染可促进淋球菌的男向女传播

  6. Performance Characteristics of Ng qPCR淋球菌感染定量分析-qPCR结果与CFU线性相关 Ng strain FA1090 Primers:- papTM-F 5' CAGCATTCAATTTGTTCCGAGTC 3' papTM-R 5' Biotin-GAACTGGTTTCATCTGATTACTTTCCA 3' papTM-probe 5' CGCCTATACGCCTGCTACTTTCACGC 3’

  7. Transmission of Ng to Women Related to Numbers of Ng in the Male Sex-Partner ANDInfluence of Pre-Existing Ct Infection in Women on Susceptibility to Ng Infection 淋球菌男向女转播效率与感染男性的淋球菌量和女性性伴是否原有沙眼衣原体感染相关 P<0.006 N=-25 5/7* women ex- posed to ≤120 cfu/ml Ng had Pre-Existing Ct infection N=-12 2/12* women Had Pre-Existing Ct infection _________ *P=0.04 (Fisher Exact Test); correlating transmission of low level exposure to Ng (≤120 cfu/ml) infection with Underlying Ct infection in women

  8. Summary • Ng transmission was enhanced from men to women with pre-existing Ct infection 女性已有沙眼衣原体感染可促进淋球菌由男向女的传播 • Men with low level Ng infection (≤120 cfu/ml equivalents in urine) failed to transmit Ng infection unless Their Female sex-partner had pre-existing Ct infection: 5/7 (71%) of women with pre-existing Ct infection exposed to low level Ng infection were infected 男性感染的淋球菌量低导致淋球菌的传播失败，除非女性性伴原已感染了沙眼衣原体，原已感染沙眼衣原体女性暴露于感染少量淋球菌的男性，有71%获得感染

  9. Animal Studies

  10. Experimental Design: Mouse Model of co-Infection (Cm and Ng) 实验小鼠共感染模型 Antibiotics (Vancomycin + Streptomycin) Inoculate C.muridarum 3.5 x 105 IFU Estrogen DAY Timing of procedures Serum sample obtained to determine Ng and Cm specifc antibodies -4 -3 -2 -1 0 1 2 3 4 5 6 7 8 9 10 Ng (FA1090) inoculation 2 x 105 CFU Vaginal swabs daily for: (1) Cm quantitative PCR, daily (2) Ng quantitative cultures, daily (3) Number of complement component 3 (C3) and Ig molecules bound per Ng organism Serum measurements of Cm- and Ng-specific antibody (whole cell ELISA), DAY 11

  11. Co-Infection of Female Mice with N. gonorrhoeae (Ng) and C. muridarum (Ct) 共感染与单独感染小鼠的病原体体内生存/清除时间和存活菌量分析 p=0.0022 100 p=0.96 % Infected 75 Mice Groups 50 % Infected Cm – Ng D 25 A Ng 0 100 - 2 - 1 0 1 2 3 4 5 6 7 8 9 75 Cm DAYS 50 25 5 0 4 p=0.96 3 Log10 IFU equiv. 8 1 2 3 4 5 6 7 8 9 10 B E 2 6 p=0.029 DAYS 1 Log10 CFU 4 0 - 2 - 1 0 1 2 3 4 5 6 7 8 9 2 0 DAYS 1 2 3 4 5 6 7 8 9 10 DAYS 40 35 p=0.45 p=0.0002 30 C 30 F AUC log10 IFU equiv. 20 25 AUC log10 CFU 10 20 15 0 Cm - Ng Cm Cm - Ng Ng GROUPS GROUPS

  12. Number (No.) C3 and Ig Molecules per Ng Organism 阴道局部C3和Ig比较方法 - C3 或 Ig 分子浓度/ 淋球菌量 Mice Groups Cm – Ng Ng 8 p=0.029 6 Log10 CFU 4 2 • Vaginal Samples from Day 1-3 (and Day 4-6) from each mouse were pooled • Ng captured to an ELISA plate coated with rabbit anti-Ng • Number of Ng organisms captured determined using an Ng mAb (calibrated) • Amount of C3, IgG, IgM and IgA bound to captured Ng determined • Density of C3, IgG, IgM and IgA (number of molecules per org.) determined 0 1 2 3 4 5 6 7 8 9 10 DAYS No. of molecules Ng organism = The Journal of Infectious Diseases 1999;179:124–35

  13. Number of C3 and Ig molecules per org Sample - Pool of vaginal washes from Day 1-3 of each mouse 采集自感染1~3天的阴道冲洗液（C3 或 Ig / 菌量）

  14. Ng and Cm Specific Antibodies (Whole Cell ELISA) in Mouse Serum, 10 days after Ng inoculation 病原体共感染与单独感染血清特异性抗体的比较 P= 0.005 P= 0.0026 P= <0.0001 ns ns ns Mouse groups (N=10 per group)

More Related