1 / 10

Understanding Bioinformatics Databases for DNA and Protein Sequences

Learn about basic bioinformatics databases, like NCBI, for DNA and amino acid sequences. Explore how to query and interpret results for specific proteins like elastase in Homo sapiens. Discover the importance of conserved domains and protein folding processes.

lyre
Download Presentation

Understanding Bioinformatics Databases for DNA and Protein Sequences

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. On line (DNA and amino acid) Sequence Information Lecture 9

  2. Introduction • Basic bioinformatics Databases • NCBI home page • Query and return results • DNA sequence results page • Protein sequence results page

  3. Bioinformatics Database • Bioinformatic Databases contain information forvarious biological data: • Consider the following [NCBI] query: • What is the DNA and amino acid sequence for the following protein: • homo sapiens[organism] AND elastase[title] AND leukocyte[title]

  4. NCBI : Entrez (main page)

  5. The nucleotide results returned

  6. The linear DNA option:5292 bp

  7. B.P. and AA sequence Note p08246 [protein code] and Base 1287 [highlighted in blue] is start codon ATG

  8. Fasta file for protein (p08246)

  9. Graphic file for protein (p08246) CDD: conserved domains; disulphide bonds between to cystine AA is important in the protein folding process: 1’ to 2’ to 3 ‘

  10. Main bioinformatics databases • DNA (nucleic acid) sequences: • NCBI(usa){ leukocyte elastase gene}, Graph format • EMBl (europe) { leukocyte elastase gene}, • Genome Databases: Ensemble elastase record • UniProt protein sequences: Elastase record • More information in the January issue of • Nucleic Acid Research (open access)

More Related