AtIAA1. AtIAA2. SlIAA2. AtIAA33. AtIAA3. SlIAA33. SlIAA3. SlIAA35. SlIAA22. 86. AtIAA29. AtIAA4. 22. SlIAA29. 12. 48. SlIAA4. 18. 78. SlIAA36. SlIAA1. 74. 60. SlIAA23. AtIAA34. 90. 12. SlIAA21. 71. AtIAA32. 97. 56. 91. SlIAA8. SlIAA32. 99. AtIAA8. AtIAA30. 19.
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Supplemental Figure S1.Evolutionary relationships of Arabidopsis and tomato Aux/IAA proteins.
The evolutionary history was inferred using the Neighbor-Joining method (Saitou and Nei, 1987). The optimal tree with the sum of branch length = 7.80811039 is shown. The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling, 1965) and are in the units of the number of amino acid substitutions per site. The analysis involved 54 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 68 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 (Tamura et al., 2011).
Supplemental Figure S2. Genomic distribution of Aux/IAA genes on tomato chromosomes.
The arrows next to gene names show the direction of transcription. The number in parentheses designates the position of the ATG of each Aux/IAA gene in megabases (Mb) on tomato chromosome pseudomolecules (Tomato Whole Genome Scaffolds data V2.4). The chromosome numbers are indicated at the top of each bar.
Supplemental Figure S3. Amino acid sequences for IAA proteinsthat contain an atypical domain I
(A) Amino acid sequences for IAA proteins that contain an expanded repression domain I. Amino acid sequence comparison of tomato Sl-IAA11, Sl-IAA12 and Sl-IAA13 with those from Arabidopsis AtIAA10 (AT1G04100), AtIAA11 (AT4G28640), AtIAA12 (AT1G04550) and AtIAA13 (AT1G04550). The conserved LxLxLxLxLx motif is underlined. The sequences were aligned with Clustal X method and adjusted manually.
(B)Amino acid sequences between conserved domains I and II for IAA proteins that contain a second LxLxL motif. Amino acid sequence comparison of tomato Sl-IAA7, Sl-IAA14, Sl-IAA16 and Sl-IAA17 with those from Arabidopsis AtIAA7 (AT3G23050), AtIAA14 (AT4G14550), AtIAA16 (AT3G04730) and AtIAA17 (AT1G04250). The conserved LxLxLx motif (Domain I) and the DLxLxL motif are underlined. The sequences were aligned with Clustal X method and adjusted manually.