Selective production of acetone during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MAceT113. Katelyn McKindles Bio 381 November 29 th , 2012. Experimental Aims.
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Katelyn McKindles Bio 381 November 29th, 2012
The structural materials that plants produce to form the cell walls, leaves, stems, stalks, and woody portions of biomass are composed mainly of three biobased chemicals called cellulose, hemicellulose, and lignin. Together, they are called lignocellulose, a composite material of rigid cellulose fibers embedded in a cross-linked matrix of lignin and hemicellulose that bind the fibers.
Lignocellulose material is by necessity resistant to physical, chemical, and biological attack, but it is of interest to biorefining because the cellulose and hemicellulose can be broken down through a process called hydrolysis to produce fermentable, simple sugars. Lignocellulosic biomass is often a waste material of the food processing and forest products industries that may be locally, readily available at low cost.
Biomass gasification is a high-temperature process (600 to 1000oC) to decompose the complex hydrocarbons of biomass into simpler gaseous molecules, primarily hydrogen, carbon monoxide, and carbon dioxide.
Clostridium species are Gram-positive, rod-shaped, spore-formers. These generally obligate anaerobes are ubiquitous saprophytes or part of our normal flora.
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Expression of thio, pta, thio + hmgCoAs and aldh in Clostridium
sp. MAceT113 (rtPCR). Lines: 1 – 1 kb Ladder; rtPCR products
for mRNA: thio (2), pta (3), thio + hmgCoAs (4) and aldh (5).
Single plasmid recombinants were detected at 7.2±0.11*10-6 per non-transformed cell, while double plasmid recombinants were detected at 8.3±0.02*10-7 per single plasmid recombinant.
Single stage continuous syngas fermentation using strain Clostridium sp. MAceT113 producing acetone.
Single stage continuous syngas fermentation usingstrainClostridium sp. MT896 producing acetate and ethanol
ALDH- acetaldehyde dehydrogenase
CoAT- acetoacetyl-CoA transferase
R16- acetoacetate decarboxylase
Found in Eukaryote cells