1 / 7

Commercial Assays and Detection of Parvovirus B19 genotypes

Commercial Assays and Detection of Parvovirus B19 genotypes. Dr Sally A. Baylis, Division of Virology NIBSC. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region. A6. Servant et al ., J. Virol. 2002. Roche Parvovirus B19 Quantification Kit. F1). -. Genotype 1.

lowellg
Download Presentation

Commercial Assays and Detection of Parvovirus B19 genotypes

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Commercial Assays and Detection of Parvovirus B19 genotypes Dr Sally A. Baylis, Division of Virology NIBSC

  2. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

  3. Roche Parvovirus B19 Quantification Kit F1) - Genotype 1 Fluorescence (F2/Back Fluorescence (F1/F2) Genotype 2 NTC Genotype 3 M 1 1 3 3 2 2 NTC M Cycle Number Cycle Number Region amplified: NS1

  4. F1) - Fluorescence (F2/Back Cycle Number Artus RealArtTM Parvo B19 LC kit Genotype 3 Genotype 2 Genotype 1 NTC M 1 1 3 3 2 2 NTC M Region amplified: VP1

  5. Sensitivity of Detection of Different Genotypes of Parvovirus B19

  6. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

  7. Summary • Choice of assay will affect outcome in terms of detection/quantification of different genotypes of parvovirus B19 • Choice of primers (and probe) effects ability to detect & quantify variant viruses • Maintain watching brief of databases to ensure adequate detection – science moves on K. Hokynar et al. 2004 J. Clin. Microbiol. S. Baylis et al. 2004 J. Virol. Methods S. Braham et al. 2004 J. Clin. Virol. B. Cohen et al. 2006 J. Clin. Virol.

More Related