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Parvovirus B19 Assay Selection: Impact on Genotype Detection and Quantification

Understand the significance of assay choice in detecting and quantifying Parvovirus B19 genotypes. Learn how primer selection affects the ability to detect variant viruses and stay updated with evolving scientific methods. Explore Roche and Artus RealArtTM kits for accurate detection.

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Parvovirus B19 Assay Selection: Impact on Genotype Detection and Quantification

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  1. Commercial Assays and Detection of Parvovirus B19 genotypes Dr Sally A. Baylis, Division of Virology NIBSC

  2. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

  3. Roche Parvovirus B19 Quantification Kit F1) - Genotype 1 Fluorescence (F2/Back Fluorescence (F1/F2) Genotype 2 NTC Genotype 3 M 1 1 3 3 2 2 NTC M Cycle Number Cycle Number Region amplified: NS1

  4. F1) - Fluorescence (F2/Back Cycle Number Artus RealArtTM Parvo B19 LC kit Genotype 3 Genotype 2 Genotype 1 NTC M 1 1 3 3 2 2 NTC M Region amplified: VP1

  5. Sensitivity of Detection of Different Genotypes of Parvovirus B19

  6. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

  7. Summary • Choice of assay will affect outcome in terms of detection/quantification of different genotypes of parvovirus B19 • Choice of primers (and probe) effects ability to detect & quantify variant viruses • Maintain watching brief of databases to ensure adequate detection – science moves on K. Hokynar et al. 2004 J. Clin. Microbiol. S. Baylis et al. 2004 J. Virol. Methods S. Braham et al. 2004 J. Clin. Virol. B. Cohen et al. 2006 J. Clin. Virol.

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