ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant) LB films containing 0 mol\% (A, C) and 30 mol\% (B, D) cholesterol-d7. M255 corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7.
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LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255
corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7.
Images E, F, G, H were obtained by subjecting A, B, C, D, respectively to a smoothing
function available as part of the IonTof software, and show more clearly the location of
the palmitate group and deuterium. Images are 50 x 50 mm2, 256 x 256 pixels with
30 mol% d7-chol
This set of images is published in the Biophysical Journal. The goal of that work was to determine the function of cholesterol in an animal extracted surfactant (BLES). TOF-SIMS was used to determine the location of cholesterol in an LB-film of BLES. Courtesy of Eleonora Keating, Univ. of Western Ontario
gold –coated mica at p = 30 mN/m. Mass 50 corresponds to C3D7 which is specific to d31-POPG,
masses 104 and 184 correspond to choline and phosphocholine which are specific to POPC respectively,
masses 108 and 188 correspond to d4-choline and d4-phosphocholine specific to d4-DPPC and mass
739 corresponds to d4-DPPC-H+. These images show that POPC and d31-POPG are present in the LE
phase while d4-DPPC is highly concentrated in the LC phase. Images are 50 x 50 mm2, 256 x 256 pixels
with 1 shot/pixel.
This set of images shows that TOF-SIMS can be used to determine the location of each phospholipid in an LB film of a mixture. In this work the authors are trying to design a synthetic surfactant that will eventually replace animal extracted BLES which is currently administered to premature babies throughout Canada.
Courtesy of Eleonora Keating, Univ. of Western Ontario