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Clarity User Training Advanced

Clarity User Training Advanced. Clarity User Training. For advanced users. User settings and display options Sequences Multidetector systems Export data Report setup Batch data re processing. For advanced users. Data archivation Column Performance Noise and Drift

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Clarity User Training Advanced

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  1. Clarity User TrainingAdvanced Clarity User Training

  2. For advanced users • User settings and display options • Sequences • Multidetector systems • Export data • Report setup • Batch data reprocessing

  3. For advanced users • Data archivation • Column Performance • Noise and Drift • Special calculations • Control modules • GLP a CFR21part11

  4. User settings User settings are stored in individual users desktop *.dsk files. For this reason they are set in the instrument window and not in the main window

  5. Labels and display options Overlay activation Math operations Moving and Scaling of Chromatograms Text Label and Lines for legends and arrows Chromatogram/Create Label

  6. Colored peak area in chromatogram When these peaks are identified in chromatogram they will be automatically colored Peaks in calibration can have a color assigned.

  7. Colored peak area in chromatogram • Highlight currently selected peaks Simply select a peak in the chromatogram result table. The result table selection overrides the color defined in calibration

  8. Colored peak area in chromatogram Use Peak Color set in Calibration file Highlight peaks selected in Results table (signal color used)

  9. Table customizing options Context menu invoked by right mouse click in table

  10. Common columns in Summary table • Independent setting of columns • Common - for all compounds • Summary – compound specific

  11. Summary table options

  12. Graph display options Context menu invoked by right mouse click in Chromatogram window Axis properties setting Graph properties setting

  13. Chromatogram display options Setting for individual signals Gradient display options

  14. Customizing Toolbars Reset All restores original settings

  15. Sequence table • Define a serie of samples to be measured • Essential for working with autosamplers • For controled autosamplers the Vial number and Injection volume are used from this table • Allows modifications during a run for not yet processed samples • Simply add samples by filling any field in the last empty row. Other fields will be copied from previous. Several samples can be added by copying from a table • Allows for import of sample data from text files • Allows reprocessing of last measured sample set

  16. Sequence table window File name, automatic %variables for SV, EV, IV Automatic recalibration from sequence Individual Post-Run settings for each row Start, End vial and Injections number. Several vials and/or injections defined on single row Tooltip with actual file name Use row, add new rows during sequence run Row status indicator

  17. Sequence options Pasive sequence Clarity only expects Start signal, analysis time is set on autosampler Active sequence Clarity sends a Ready signal to autosampler and waits for Start signal AS control (Active sequence) Autosampler injects according to SV, EV, IV and Inj. Volume in sequence table Initial value for %n Editation of template method used on selected row

  18. Sequence control Run sequence starts complete sequence (resets thestatus) Resume sequence continues from last injection Pause sequence Skip Vial Stop sequence will stop the sequence run. Repeated Stop will stop the current analysis Repeat Injection Status of the sequence. Stored at Pause, Abort, Stop Can be changed by Edit/Reset Statusfor selected rows

  19. Sequence import Select the file to import Assign the respective fields to be imported Only Start vial (SV) and file name must be set (marked in bold) Other fields will be filled by default values

  20. Export data • Export data to other programs to prevent typing errors • Simple Copy and Paste (Ctrl C – Ctrl V) • Export Data settings common for • manual export from chromatogram window • automatic export from postrun at the end of each run • automatic export by batch reprocess of chromatograms or sequence • Export of chromatograms in common formats

  21. Export data - simplest Copy a picture to MS Office (including labels) via Clipboard or store it in *.emf format Copy a selected part of table using Copy (Ctrl C) and Paste (Ctrl V)

  22. Export data - options Export data: manual - from Chromatogram window automatic - according to Setting/Postrun batch - from Analysis/Batch Export time/signal data pairs (import to other programs – for example graphics) Headers: Column headers and chromatogram info Full format: Filename, date and time before each row blank field = actual chromatogram name + .txt extension Path can be used All exported data are in single file

  23. Export data - example

  24. Batch processing • print • Report style from Report Setup dialogwill be used • do not forget to switch off the Overlay mode • export • reprocess of chromatograms according to instrument method • reprocess whole sequence • electronic signature Export according to Settings - Export Data File type Chromatograms Calibration standards Sequence Files Electronic signature for series of chromatograms

  25. Multidetector systems • Assignment of detectors to Clarity Instruments in the System Configuration dialog • All signals stored in a single chromatogram • Result table, Integration table and Acquisition parameters are individual for each chromatogram • Common calibration file • calibration curves are individual for each signal • Summary table for display of results from all signals

  26. Multi signal chromatograms Connecting link marks signals from one chromatogram. Click icon to set signal active Detector Delay compensates for interdetector volume, inactive for first signal Results are displayed individually, some properties like calibration are common for the whole chromatogram

  27. Overlay Ctrl + click will hide/show the signal Ctrl + click will lock the tool for repe-titive use. In overlay, several chromatograms can be compared. Maximum number can be set in user options Scale, move and math operations are active only in overlay Doubleclick on legend will set signal active Summary table displays results for several chromatograms or signals simultaneously

  28. Report Setup - logo

  29. Report Setup – tiled signals

  30. Archiving and backup • To reduce the data size compressed archive *.dgz files should be used • *.dgz archives always replace all data (files deleted from project will be during archiving deleted also from the archive). • It is advantageous to archive whole projects – should be considered when planning project structure

  31. Archive data • Archive and Restore for copying or moving • projects • Selected file types • Without compressing = original format and structure • compressed *.dgz archives = single file

  32. Performance parameters Separation parameters Advanced tab in the Method Setup (displays template method Values used for Capacity ratio and Efficiency calculation Performance tab (hidden in default layout) Calculation type

  33. Noise monitoring Actual noise monitoring in the Data Acquisition window

  34. Noise and Drift Drift and Noise can be used for in User column calculations Drift and Noise displayed for selected intervals in Result table header

  35. Calculations • ISTD calculations • Normalized % calculations • Groups • calibration of peak group like single compound • example - isomers (xylen) • Scale factor • recalculation of result to other units • Uncalibrated response • quantification of unidentified peaks using Uncalibrated response factor • Injection volume • Injected volume correction • for manual injection in GC

  36. ISTD calculations ISTD calculations are performed: a) Amount for ISTD is setin bothcalibration and sample b) both are zero ISTD compound is calibrated Only one ISTD compound is allowed in calibration. If the conditions are not met, no results will be calculated ISTD peak can be hidden in the Result table ISTD amount in sample is given

  37. Normalized % calculations Amount % gives the normalized % if all integrated peaks are calibrated and Sample amount is zero If Sample amount is non-zero, it will replace the sum in the Amount column and serve as a base for Amount % calculation Total line is calculated as a sum of all groups + sum of peaks not belonging to any group. Groups are calibrated, thus peaks should not be both calibrated and in a group to get correct Amount% values

  38. Groups 1st step Adding peaks to a group in the integration table 2nd step Setting a Group in Calibration table. The compound name and amount is set as for ordinary peaks

  39. Scale Factor and Uncal.Response Uncal. Response: Used for quantification of unidentified compounds. For universal response detectors (ELSD, FID, RI) Scale factor and Units after scaling: Multiplies values in Amount column and changes the units in column header. Method specific parameter, multiplies also the entered Sample Amount value Dilution: Multiplies values in Amount column. Sample specific parameter (entered from Sequence or Single Analysis window).

  40. Injection Volume • Default Injection Volume must be set first in calibration and Injection Volume set in standards before calibrating • Supposes linear detector response • Can lead to incorrect results, if used improperly Setting Injection Volume for sample and standard Setting Default Injection Volume in calibration

  41. Calibration Command Calibration/Show History displays data used for selected level Deviation of point from calibration curve in % Residual sum of deviation squares

  42. Retention indexes, LOD, LOQ • Calibration • Retention (Kovats) indexes • LOD and LOQ limits Columns for Retention (Kovats) indexes are hidden in default settings. Use setup columns to disdplay them Amounts lower then specified LOD or LOQ limits are marked in Peak type column

  43. Control Modules • For selected instruments the direct control is available. The advantages are • The instrument parameters are stored in a method • It is not necessary to enter the parameters twice, especially useful for autosamplers • The parameters are recorded in the chromatogram

  44. Control modules (GC) Control modules enable full control of connected devices like gradient programe and other settings. The used settings are stored within a chromatogram.

  45. Control modules (LC, AS)

  46. Control Modules • Option to Send/Confirm/Do not send method to instruments after method change Confirmation dialog Send Method options

  47. Control modules overview

  48. Additional control • Other devices can be controlled by A/D board digital outputs: • gradient or detector program start • detector autozero • valve switching • fraction collector control • Synchronization in Active Sequence • Dig. output set in System Configuration, not in the Event table

  49. Event table • Set events in the Event Table tab in the Method Setup • Named events • Digital event input type • Output event can trigger run/sequence commands: • Stop • Abort • Skip vial • Shutdown • Repeat injection

  50. GLP a CFR21 part 11 • Chromatogram history • All saved versions are stored in the chromatogram • Versions can be opened (including calibration) • Audit trails • Station, Chromatogram, Sequence, Calibration • User access • Can be restricted by User Accounts settings

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