1 / 6

Exploring Protein Structures: Bioinformatics Fundamentals

Dive into classical structural biology methods to determine, purify, and analyze protein structures, alongside exploring genomic DNA sequences for predicting and understanding protein mechanisms and functions. Learn how structural genomics aims to cover a wide sequence space and why protein function is not solely dependent on sequence identity. Discover the fundamental principles of protein structures, from primary sequences to quaternary arrangements, with a focus on helices, sheets, and multisubunit structures.

linda-downs
Download Presentation

Exploring Protein Structures: Bioinformatics Fundamentals

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Protein structure Friday, 10 February 2006 Introduction to Bioinformatics Brigham Young University DA McClellan david_mcclellan@byu.edu

  2. Classical structural biology Determine biochemical activity Purify protein Determine structure Understand mechanism, function Fig. 9.1 Page 274

  3. Structural genomics Determine genomic DNA sequence Predict protein Determine structure or analyze in silico Understand mechanism, function Fig. 9.1 Page 274

  4. Structural genomics A goal of structural genomics is to determine protein structures that span the full extent of sequence space. Page 273

  5. Protein function and structure Function is often assigned based on homology. However, homology based on sequence identity may be subtle. Consider RBP and OBP: these are true homologs (they are both lipocalins, sharing the GXW motif). But they are distant relatives, and do not share significant amino acid identity in a pairwise alignment. Protein structure evolves more slowly than primary amino acid sequence. RBP and OBP share highly similar three dimensional structures. Page 274

  6. Principles of protein structure Primary amino acid sequence Secondary structure: a helices, b sheets Tertiary structure: from X-ray, NMR Quaternary structure: multiple subunits Page 276

More Related