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ToF-SIMS Imaging of BLES Films with Varying Cholesterol-d7 Concentrations: An Analysis

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This study employs ToF-SIMS imaging to explore the spatial distribution of cholesterol in bovine lipid extract surfactant (BLES) monolayers containing 0 mol% and 30 mol% cholesterol-d7. Images focus on the localization of palmitate (M255) and deuterium (M2) from the cholesterol derivative within LB films. High-resolution images were processed using IonTof software to enhance visibility of lipid components. These findings contribute to understanding the functional role of cholesterol in lung surfactants, as published in the Biophysical Journal.

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ToF-SIMS Imaging of BLES Films with Varying Cholesterol-d7 Concentrations: An Analysis

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  1. ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant) LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255 corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7. Images E, F, G, H were obtained by subjecting A, B, C, D, respectively to a smoothing function available as part of the IonTof software, and show more clearly the location of the palmitate group and deuterium. Images are 50 x 50 mm2, 256 x 256 pixels with 1 shot/pixel. BLES + 30 mol% d7-chol BLES A B M255 D C M2 F E M255 processed G H M2 processed This set of images is published in the Biophysical Journal.  The goal of that work was to determine the function of cholesterol in an animal extracted surfactant (BLES).  TOF-SIMS was used to determine the location of cholesterol in an LB-film of BLES. Courtesy of Eleonora Keating, Univ. of Western Ontario

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