laboratory biosafety levels n.
Skip this Video
Loading SlideShow in 5 Seconds..
Laboratory Biosafety Levels PowerPoint Presentation
Download Presentation
Laboratory Biosafety Levels

Loading in 2 Seconds...

play fullscreen
1 / 28

Laboratory Biosafety Levels - PowerPoint PPT Presentation

  • Uploaded on

Laboratory Biosafety Levels. Goals. Define barriers and procedures used by laboratories to protect workers and others from infection Describe the four biosafety levels and the protective measures used by each type of laboratory when handling infectious materials

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about 'Laboratory Biosafety Levels' - liam

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
  • Define barriers and procedures used by laboratories to protect workers and others from infection
  • Describe the four biosafety levels and the protective measures used by each type of laboratory when handling infectious materials
  • Provide examples of the types of biological agents handled in each type of laboratory
  • Describe typical places where each type of laboratory can be found in the US
why is biosafety important
Why is Biosafety Important?
  • Laboratorians recognize hazards of processing infectious agents
  • Guidelines developed to protect workers in microbiological and medical labs through engineering controls, management policies, work practices
biosafety levels
Biosafety Levels
  • Precautions so people researching or trying to identify organisms do not become infected
  • While handling or testing clinical specimens, workers could accidentally infect themselves or coworkers
  • Labs must adhere to very specific safety regulations to work with organisms that pose a threat to human health
biosafety levels1
Biosafety Levels
  • Regulations outline precautions, special practices, decontamination procedures
  • Labs divided into 4 biosafety levels; protective practices increase with each
    • Biosafety Level 1 labs - work with least dangerous agents, require fewest precautions
    • Biosafety Level 4 labs - have strictest methods because dealing with agents that are most dangerous to human health
about this information
About this Information…
  • Information summarized here should not be used to establish laboratory safety protocols
  • Complete information and recommendations can be found in Biosafety in Microbiological and Biomedical Laboratories 5th Edition at bmbl4s6.htm
  • Primary barriers: physical barriers or personal protective equipment between lab worker and pathogen
    • Gloves, masks, special breathing apparatuses
  • Secondary barriers: structural aspects of the laboratory that make working environment safer against infection
    • Sinks for handwashing, special containment areas, special air ventilation patterns 
universal precautions
Universal Precautions
  • Universal precautions developed to protect health professionals
    • Most often apply in a clinical setting
    • May also be important for field epidemiology practices during an outbreak investigation (e.g., collecting lab specimens)
  • Include hand hygiene, gloves, gown, masks, eye protection, face shields, safe injection practices
  • Require that all equipment or contaminated items are handled to prevent transmission of infectious agents
  • Special circumstances may require additional precautions
    • Protective clothing, special site decontamination
biosafety level 1 bsl 1
Biosafety Level 1 (BSL-1)
  • Agents not known to cause disease in healthy adults
    • Some organisms may cause disease in immunocompromised individuals
  • Agents include Bacillus subtilis, Naegleria gruberi, infectious canine hepatitis virus, non-pathogenic E. coli species

(transmission electron micrograph of E. coli)

biosafety level 1 bsl 11
Biosafety Level 1 (BSL-1)
  • Standard practices required:
    • frequent handwashing
    • door that can be kept closed when working;
    • limits on access to the lab space when working;
    • no smoking, eating, drinking, storage of food in laboratory;
    • care to minimize splashes and actions that may create aerosols (tiny droplets);
    • decontamination of work surfaces after every use after any spills;

(continued on next slide)

biosafety level 1 bsl 12
Biosafety Level 1 (BSL-1)
  • Standard practices (continued):
    • decontamination of laboratory wastes;
    • use of mechanical pipettes only (no mouth pipetting);
    • "sharps" precautions, including special containers for disposing of needles and other sharp objects;
    • maintenance of insect/rodent control program;
    • use of personal protective equipment (lab coats, latex gloves, eye protection or face shields)
  • Open bench top sink for hand washing
biosafety level 2 bsl 2
Biosafety Level 2 (BSL-2)
  • Agents associated with human disease
    • Generally required for any human-derived blood, bodily fluids, tissues in which infectious agent may be unknown
  • Agents include measles virus, Salmonella species, pathogenic Toxoplasma, Clostridium botulinum, hepatitis B virus

(transmission electron micrograph of hepatitis B virus)

biosafety level 2 bsl 21
Biosafety Level 2 (BSL-2)
  • Primary hazards:
    • accidental needle sticks
    • exposure to eyes and nose (mucous membranes)
    • ingestion of infectious materials
  • Agents do not cause lethal infections, are not transmissible via airborne route
    • (do not cause infection if tiny droplets become airborne and are inhaled, which might occur if the material were spattered)
  • Agents are pathogens for which immunization or antibiotic treatment is available
  • Extreme care should be taken with contaminated needles and sharp lab instruments
biosafety level 2 bsl 22
Biosafety Level 2 (BSL-2)
  •  Standard practices include BSL-1 plus:
    • policies to restrict access to lab;
    • biohazard warning signs posted outside lab;
    • surveillance of laboratory personnel with appropriate immunizations offered;
    • biosafety manual with definitions of needed waste decontamination or medical surveillance policies;
    • supervisory staff who have experience working with infectious agents and specific training for laboratory personnel in handling these agents
biosafety level 2 bsl 23
Biosafety Level 2 (BSL-2)
  • Primary barriers: biosafety cabinets or other approved containment devices
  • Personal protective equipment: lab coats, gloves, face protection as needed
  • Protective clothing removed when personnel leave laboratory area
  • Cabinets thoroughly decontaminated daily and monitored for radiation for personal protection
  • Secondary barriers: BSL-1 barriers plus autoclave for glassware
biosafety level 2 bsl 24
Biosafety Level 2 (BSL-2)
  • Example of biosafety sign posted outside lab working with infectious agents

Lab’s biosafety level

Infectious agents under study

Contact information for responsible person and 2 emergency contacts

biosafety level 3 bsl 3
Biosafety Level 3 (BSL-3)
  • Agents with potential for respiratory transmission, may cause serious and potentially lethal infection
    • May be studied at BSL-2 for diagnosis 
  • Agents include Mycobacterium tuberculosis,St. Louis encephalitis virus, Francisella tularensis, Coxiella burnetii

(F. tularensis under direct fluorescent antibody stain)

biosafety level 3 bsl 31
Biosafety Level 3 (BSL-3)
  • Primary hazards: needle sticks, ingestion, exposure to infectious aerosols
  • For example:
    • Public health surveillance for West Nile virus includes testing birds
    • In August 2002, state laboratory worker cut finger while dissecting bird; 4 days later, had symptoms of fever, myalgia, recurring sweats, hot flashes
    • Worker and bird both diagnosed with West Nile
    • 2 other lab-acquired cases in 2002
biosafety level 3 bsl 32
Biosafety Level 3 (BSL-3)
  • Tularemia common source of laboratory-acquired infection
    • infections occur while handling infected animals or experimenting with cultures
    • Laboratory-acquired infections known to occur but not reportable before 9/11/2001
    • Tularemia now classified as potential biological weapon
biosafety level 3 bsl 33
Biosafety Level 3 (BSL-3)
  •  Standard practices include BSL-2 plus:
    • strictly controlled access to the lab;
    • specific training for lab personnel in handling potentially lethal agents;
    • decontaminating all waste;
    • changing contaminated protective lab clothing, decontaminating lab clothing before laundering;
    • institutional policies regarding specimen collection and storage from workers to establish exposure
biosafety level 3 bsl 34
Biosafety Level 3 (BSL-3)
  • Primary barriers:
    • Similar to BSL-2 personal protective equipment
    • Respiratory equipment if risk of infection through inhalation
  • Secondary barriers:
    • All BSL-2 barriers
    • Corridors separated from direct access to lab
    • Access through self-closing double doors
    • Air handling systems to ensure negative air flow (air flows into the lab)
    • Air pumped into lab not re-circulated in building
biosafety level 4 bsl 4
Biosafety Level 4 (BSL-4)
  • Dangerous and exotic agents with high risk of life-threatening disease, aerosol-transmitted
  • Related agents with unknown risk of transmission
  • Agents (all viruses) include Marburg virus, Ebola virus, viruses that cause Congo-Crimean hemorrhagic fever, Lassa fever

(transmission electron micrograph of Ebola virus)

biosafety level 4 bsl 41
Biosafety Level 4 (BSL-4)
  • Primary hazards:
    • respiratory exposure to infectious aerosols
    • mucous membrane exposure to infectious droplets
    • accidental sticks with needles or other sharp objects contaminated with infectious material
  • For example
    • In late 1960s, 25 laboratory-acquired Marburg infections, including 5 deaths
    • Workers studying infected monkeys from Uganda
    • First documented naturally-occurring human case occurred in 1975
biosafety level 4 bsl 42
Biosafety Level 4 (BSL-4)
  • Personnel must receive specialized training in handling extremely dangerous infectious agents, containment equipment and functions
  • Access to lab is restricted: immunocompromised persons are never allowed to enter the lab
  •  Standard practices include BSL-3 plus:
    • strictly controlled access to the laboratory;
    • changing clothing before entering and exiting lab (showering upon exiting recommended);
    • decontaminating all material exiting facility
biosafety level 4 bsl 43
Biosafety Level 4 (BSL-4)
  • Primary barriers:
    • Biosafety cabinets used at other biosafety levels
    • Full-body, air-supplied, positive pressure personnel suit
  • Secondary barriers:
    • All physical barriers at BSL-3
    • isolated zone or a separate building;
    • dedicated supply and exhaust, vacuum, decontamination systems;
    • a recommended absence of windows (or sealed and resistant to breakage)
laboratory locations
Laboratory Locations
  • BSL-1: high schools, community colleges, municipal drinking water treatment facilities
  • BSL-2: local health departments, universities, state laboratories, private laboratories (hospitals, health care systems), industrial laboratories (clinical diagnostic companies)
  • BSL-3: state health departments, universities, private companies, industry, federal government (NIH, CDC)
  • BSL-4: only 15 facilities in the US
    • 9 federal (CDC, NIH), 4 university (Georgia State University, University of Texas Medical Branch), 1 state, 1 private
    • Renovations underway at several labs, new facilities proposed at additional sites
  • Laboratorians have long recognized hazards of processing infectious agents
  • Biosafety guidelines developed to protect workers in microbiological and medical labs through a combination of safeguards including engineering controls, management policies and work practices.
  • Issue described differences between biosafety levels
  • Help you understand process labs may have to undertake to identify microorganism, why every lab cannot test for every organism
  • US Department of Health and Human Services, Centers for Disease Control and Prevention and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: US Government Printing Office; 2007. biosfty/bmbl5/bmbl5toc.htm. Accessed February 6, 2008.
  • Clemson University Environmental Health and Safety. Biological Safety [online training]. Accessed February 6, 2008.
  • Centers for Disease Control and Prevention. Laboratory-Acquired West Nile Virus Infections — United States, 2002. MMWR Morb Mort Wkly Rep. 2002; 51:1133-1135. mmwrhtml/mm5150a2.htm. Accessed February 6, 2008.
  • United States Government Accountability Office. High-Containment Biosafety Laboratories: Preliminary Observations on the Oversight of the Proliferation of BSL-3 and BSL-4 Laboratories in the United States. Publication GAO-08-108T. abstract.php?rptno=GAO-08-108T. Published October 4, 2007.