Biological Safety Training. Radiological and Environmental Management Environmental Health Purdue University October 2003. Institutional Biosafety Committee (IBC) Procedures.
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Radiological and Environmental Management
Go to: IBComm@purdue.edu to contact the committee.
EXPOSURE to biohazards may occur from:
Principal Investigators who are unsure if a material is a biohazard should contact the University Biosafety Officer, firstname.lastname@example.org
Biosafety levels corresponds to the Class rating of the biohazard, i.e., BSL2 is required for Class II biohazards. BSL2 is the most common type of biohazard project that occurs on campus. Labs working under these conditions must have the following procedures in place:
A typical selection of PPE for biohazard application
would be the use of nitrile or latex gloves, goggles, lab
coat, closed toe shoes, and perhaps a N-95 dust and
Respiratory Protection comes in many varieties. Choose the appropriate mask for the task.
BIO-RELATED WASTE MATERIALS:
When handling a bio waste bag, be careful not to press the bag against your body. This prevents improperly disposed of sharp accidents. Do not create aerosols by forcing the air out of the waste bag.
Hold Bio bags away from your body.
Don’t squeeze the bag so that aerosols are released into your breathing space.
Take biowaste to the autoclave room as soon as possible.
- Sealed containers - Aerosol cans
- Volatiles - Uncontained sharps
Laboratory employees that routinely work with sharps and building services personnel who handle wastes are at risk of being punctured or lacerated during their workday.
Report sharp related injuries to your supervisor and to the Biosafety Officer immediately.
Laminar Flow Clean Benches
For more instructions see Purdue’s Biological Safety Manual
REPORT ALL SPILLS TO YOUR SUPERVISOR AND THE BIOSAFETY OFFICER
Use the proper PPE
Use the proper disinfectant solution.
Dispose of clean-up materials in biohazard bags.
Many of these disinfecting agents can be irritating and toxic and should be used in accordance with label direction for personal protective equipment, concentration, and contact time.
Special security measures apply to users of Select Agents. See the Biosafety Officer for more specific details.
Center For Disease Control and Prevention
Select Agent Program
Biosafety in Microbiological and Biomedical Laboratories 4th Edition, May 1999
Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines)
Material Safety Data Sheets For Research Pathogens
Purdue University Biosafety Manual
Please take the following review quiz, then check your answers on the last slide.
1) Why is it important for lab staff to have bio-
agent awareness training?
a) Some agents can cause serious complications for pregnancies and immuno-compromised individuals.
b) Signs and symptoms can determine if an exposure has taken place.
c) De-con procedures can be determined at that time.
d) All of the above.
2)While working with an organism that has an exposure route including skin contact and respiratory, what personal protective equipment (PPE) is needed?
a) Biosafety cabinet
b) Respiratory or N-95 mask
c) Protective outer wear, gloves and goggles
d) All of the above
3) Why is posting a Hazard Assessment important ?
a) It is an accessible information source for employees to
use to determine proper personal protective equipment.
b) It is required by the Center For Disease Control.
c) It is an ineffective bureaucratic hoop to jump.
b) Open toed shoes
5) Sharps are defined as anything that will cut, puncture, or abrade the skin.
6) What should be done in the event of a bio-exposure sharps injury?
a) Wash the site with soap and water
b) Inform your supervisor
c) Contact REM
d) All of the above
b) disposed of in the regular trash.
c) held against your body or squeezed
d) All of the above
8) If a spill occurs inside of a Biological Safety Cabinet, which of the following statements is not appropriate procedure to follow?
a) Report the spill to your supervisor immediately.
b) Clean it up fast, before anyone sees it.
c) Wear the proper Personal Protective Equipment for the material for which you are working.
d) Plan in advance for an emergency. For example, what supplies and equipment should you maintain in your area to assist you in the event of an accidental spill?
a) Purdue Police
b) The supervisor
c) The Building Deputy
d) Everyone is responsible for security in the laboratory.
10) Who should be contacted for questions about Biosafety related issues?
a) University Police
b) Biosafety Officer
c) Building Deputy
d) Radiation Safety Officer
1) D 8) B
2) D 9) D
3) A 10) B