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DVT-IMP Low cost generic diagnostic platform. Low-cost generic diagnostic platform. Label-free ligand/receptor binding using immobilised bioreceptor on impedimetric transducer Suitable for a broad range of detection Bacteria Virus Aptamers and DNA/RNA hybridization

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DVT-IMPLow cost generic diagnostic platform


Low cost generic diagnostic platform l.jpg
Low-cost generic diagnostic platform

  • Label-free ligand/receptor binding using immobilised bioreceptor on impedimetric transducer

  • Suitable for a broad range of detection

    • Bacteria

    • Virus

    • Aptamers and DNA/RNA hybridization

  • Suitable for a broad range of sample

    • No sample pre-treatment required (whole blood)

    • Biological fluids

    • Aqueous samples

  • Rapid testing (< 5 mn)

  • Sensitivity: Large dynamic range: 0.1 pg/mL to 1 µg/mL


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Main achievements

Sampling of blood sample on transducer

Incubation & ligand/receptor binding

Washing

Detection

  • Detection of D-dimer has been demonstrated in whole blood from a patient

  • Results were matching those obtained with conventional bench-top system (Pathfast)

  • Ultra-low level of non-specific binding

  • Large dynamic range: 0.1 pg/mL to 1 µg/mL

    (larger using CNT or Gold nanoparticles)

  • TotalTime for analysis (theory <5 mins)

  • Potential of multiplexing diagnostics

  • E-Health enabled portable Reader

Nyquist Plots for varying model [Ag]

Dynamic range: 100pg/ml – 100ng/ml, Opt. temp. 250C


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Diagnosing DVT

  • D-dimer is accepted as a clinical biomarker of recent clotting

    • It is one of the smallest antigens found in human and animal blood

    • It results from the degradation of cross-linked fibrin formed during clotting and is hence indicative of clotting

    • The critical diagnostic concentration level of D-dimer is 500 ng/mL in whole blood.

      • The critical level used does vary from clinic to clinic

  • For patients with low risk factors for DVT, a normal d-dimer levels can be used to exclude DVT


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Applications

This generic platform is compatible with multiplex testing.

5 analysis can be done in parallel

More analysis could be performed if required.

It opens the field to a broad range of applications:

  • POC diagnostic

  • Environmental, Food and Water monitoring

  • Defence and Security

    • Pre-incident screening (e.g. detection of explosives, drugs, toxins, bio warfare agents at airports etc)

    • Post-incident forensic analysis and diagnostics


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High level team and competencies

  • Expertise in core development team

    • Prof. Z. Ali – Head centre for Nano & Microsystems

    • Dr V. Auger – Microfabrication for electrochemical & bio analysis

    • Dr S. Scott – Simulation and mechanical engineering

    • Dr C. Lee – Microfabrication and medical devices

    • Mr A. Lakey – Impedimetric biosensors

  • Additional expertise from consortium

    • Fraunhofer IZM (DE) – Roll-to-Roll

    • PCB (SP) – Microfabrication of film

    • Paris-Sud and UCBL (FR) – Functionalisation of impedimetric electrodes

    • Bratislava (CZ) – Clinical analysis with ethical approval granted

    • Budapest (HU) – Impedimetric instrumentation

  • Potential development partners in foundry and diagnostics areas


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A flexible collaboration for you:

  • a technology to be integrated to your internal developments throughIP licensing on applications

  • setting-up of co-development projects both at the component and system level

  • a plug and play solution: consumable and/or associated system tailored to your target market specifications

    We are very interested in your point of view regarding such technologies and development.

    Please do not hesitate to ask for additional information.