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The Well-Connected Cell

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  1. The Well-Connected Cell Text and image sources are included using the notes function of this file

  2. Classes of cell-cell junctions

  3. ZO-1ZO-3 DNA Tight (Occluding) Junctions Sealers Organizers Informants Claudins Occludins JAMs ß-catenin PTEN

  4. Tight Junctions seal dense tracer

  5. Tight Junction interactome

  6. Anchoring junctions strengthen

  7. I. Belt (Adherens) Junctions in cardiac muscle Cadherin CAMs

  8. Belt Junctions back up tight junctions

  9. The Adherens Junction interactome The Adherens Junction Interactome

  10. Forging links GFP::actin

  11. II. Desmosomal Junctions (Plakoglobins anchor MFs) CytokeratinDesmosomes

  12. A day in the life of an epithelium Desmocollin-GFP Imaged over a 24 hour period

  13. III. Hemidesmosomes anchor IMF to ECM RGD (Arg-Gly-Asp) via integrin Heterodimers (18 alpha x 8 beta) ECM

  14. Loss of hemidesmosomal connections creates blistering diseases

  15. IV. Focal Adhesion sites (FAds)link microfilaments to ECM

  16. FAd interactome

  17. Focal Adhesion Kinase (FAK) /VASp

  18. FAds anchor stress fibers Actin-GFP and Vinculin

  19. GFP::VASp localizes at FAds

  20. Special Delivery?Microtubules target FAds GFP::CLIP-170 (+TIP) and Ds-Red::zyxin (@ FAds) GFP::tubulin and Ds-Red::zyxin (@ FAds)

  21. Communicating Junctions GFP-Connexin-43 localizes at cell-cell contact points

  22. Connexin hexamers form gap junctions 2nm fields

  23. Gap junction fields Tightly-packed gap junctions

  24. Gap junctions permit low MW molecules (<1kDa) to move between cells scrape

  25. Gap junction interactome

  26. AFM of gap junction field reveals the 6-fold symmetry of the connexion channel +Ca++ -Ca++ And a role for calcium in conductance regulation

  27. Calcium Wave

  28. Electrical coupling in engineered heart muscle tissue

  29. Plasmodesmata The symplast refers to the linked protoplasmic network in plants

  30. Plant cell lineages are linked in distinct symplastic routes